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Transcriptional profiling of human naive embryonic stem cells

ABSTRACT: Transcriptional profiling of human naive embryonic stem cells

PROVIDER: PRJEB12748 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1273710_1.fastq.gz	Fastqsanger.gz
	ERR1273710_2.fastq.gz	Fastqsanger.gz
	ERR1273711_1.fastq.gz	Fastqsanger.gz
	ERR1273711_2.fastq.gz	Fastqsanger.gz
	ERR1273712_1.fastq.gz	Fastqsanger.gz

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Transcriptional profiling of human naive embryonic stem cells

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Project description:Significant interest has been devoted to the isolation of human pluripotent stem cells displaying the naive state of mouse embryonic stem cells. However, to what extent naive human cells isolated in culture resemble pluripotent cells in vivo remains unclear. Here we present three lines of evidence indicating that naive pluripotent stem cells generated in the absence of transgenes share defining molecular signatures with the human pre-implantation embryo. First, a comprehensive analysis of the transposcriptome reveals that naive human cells have a retroelement expression profile of human cleavage stage embryos. Second, base-resolution mapping of the DNA methylome in naive human cells reveals a genome-wide reduction in CpG and non-CpG methylation levels. Third, female naive cells exhibit an X chromosome status that is similar to that of the human blastocyst. Our work demonstrates that pluripotent stem cells with epigenomic hallmarks of the early human embryo can be directly captured in vitro. Examine the methylomes of 6 naÃ¯ve, 2 primed and 2 re-primed human embryonic stem cells

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