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Aspergillus niger iron study

ABSTRACT: Aspergillus niger N402 derivatives grown with different amounts of iron in the medium

PROVIDER: PRJEB20746 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1960520.fastq.gz	Fastqsanger.gz
	ERR1960521.fastq.gz	Fastqsanger.gz
	ERR1960522.fastq.gz	Fastqsanger.gz
	ERR1960523.fastq.gz	Fastqsanger.gz
	ERR1960524.fastq.gz	Fastqsanger.gz

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Aspergillus niger supplement study

Project description:Aspergillus niger N402 derivatives grown with different supplements

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Aspergillus niger WT: Control vs. xylan induction

Project description:Transcriptional profiling of the A. niger WT (N402) strain treated with xylan (1%, w/v) for 6, 12 and 24 h. The main objective was to identify genes related to cellulases and hemicellulases after treatment with the polysaccharide xylan. The experiment was further validated by enzymatic assays. Three-condition experiment: WT-Xylan for 6, 12 and 24 h at 30oC in batch culture. Firstly, the WT (N402) strain was grown in minimal medium with fructose as carbon source (control), and then transferred to 1% (w/v) xylan as carbon source. 2 biological replicates per time point.

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Project description:Galactose catabolism in Aspergillus nidulans is regulated by at least two regulators, GalR and GalX. In Aspergillus niger only GalX is present, and its role in D-galactose catabolism in this fungus was investigated. Phenotypic and gene expression analysis of a wild type and a galX disruptant revealed that GalX does not substitute for the absence of GalR in A. niger, it regulates the D-galactose oxido-reductive pathway, but not the Leloir pathway. Four genes, including the recently characterized ladB (galactitol dehydrogenase) were found to have differencial expressions that are highly relevant to GalX , indicating a novel oxido-reductive pathway in A.niger . We aim to discover differentially expressed genes in A.niger wild type strain N402 and M-NM-^TgalX mutant while growing on galactose as carbon source. Biological duplicates were made for both strains. The strains were grown O/N in complete medium with 2% frunctose and mycelium was then washed and transferred to minimal medium with 25 mM D-galactose and incubated for 2 hours. Affymetrix microarray experiments were performed RNA isolated from these samples.

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