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The Evolutionary History of Dogs in the Americas

ABSTRACT: Whole genome sequences of two canine transmissible venereal tumours (CTVTs) and their hosts derived from The Gambia.

PROVIDER: PRJEB22148 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2094835.fastq.gz	Fastqsanger.gz
	ERR2094835_1.fastq.gz	Fastqsanger.gz
	ERR2094835_2.fastq.gz	Fastqsanger.gz
	ERR2094836.fastq.gz	Fastqsanger.gz
	ERR2094836_1.fastq.gz	Fastqsanger.gz

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The Evolutionary History of Dogs in the Americas

Project description:Whole genome sequences of two canine transmissible venereal tumours (CTVTs) and their hosts derived from The Gambia.

| PRJEB22148 | ENA

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The evolutionary history of dogs in the Americas.

Project description:Dogs were present in the Americas before the arrival of European colonists, but the origin and fate of these precontact dogs are largely unknown. We sequenced 71 mitochondrial and 7 nuclear genomes from ancient North American and Siberian dogs from time frames spanning ~9000 years. Our analysis indicates that American dogs were not derived from North American wolves. Instead, American dogs form a monophyletic lineage that likely originated in Siberia and dispersed into the Americas alongside people. After the arrival of Europeans, native American dogs almost completely disappeared, leaving a minimal genetic legacy in modern dog populations. The closest detectable extant lineage to precontact American dogs is the canine transmissible venereal tumor, a contagious cancer clone derived from an individual dog that lived up to 8000 years ago.

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Project description:Transcriptional profiling of dog muscle tissue comparing control dogs. tested, genomewide, for genes differentially expressed in muscle between the escapers and the affected dogs. Using Agilent mRNA SurePrint Canine arrays, we compared muscle gene expression of the two escapers, four affected, and four normal dogs at age 2 years.

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Project description:The canine transmissible veneral tumour (CTVT) is one of the few known clonally transmissible cancers in nature. CTVT regresses spontaneously or after a single treatment with vincristine, however we know little of the mechanisms. To understand CTVT regression, we performed transcriptional analyses on serial biopsies of regressing and non-regressing CTVT, aiming to identify the likely drivers of CTVT regression.

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