ABSTRACT: EMG produced TPA metagenomics assembly of the Metagenomic analysis reveals unusually high incidence of proteorhodopsin genes in the ultraoligotrophic Eastern Mediterranean Sea (Highly abundant proteorhodopsin genes in the Eastern Mediterranean Sea) data set.
Project description:The goal of this study was to identify the key functions of the six main symbionts that are hosted in gills of the marine bivalve, Idas modiolaeformis, which lives at deep-sea hydrocarbon seeps and wood falls in the Eastern Atlantic Ocean and the Mediterranean Sea. These symbionts include the main autotrophic methane- and sulfur-oxidizing lineages (Methyloprofundus, Thioglobus, Thiodubillierella), as well as a Methylophagaceae methylotrophic autotroph, a flavobacterial degrader of complex polysaccharides Urechidicola and a Nitrincolaceae heterotroph that specializes in degradation of nitrogen-rich compounds such as peptides and nucleosides. Four I. modiolaeformis individuals were preserved in RNAlater following retrieval from a brine pool habitat in the Eastern Mediterranean at 1,150 m water depth (32° 13.4' N 34° 10.7' E), using a remotely-operated vehicle. RNAlater was discarded after 24 hours, and the specimens were kept at -80°C until DNA/RNA/protein co-extraction using the AllPrep DNA/RNA/Protein Mini Kit (Cat. No. 80004, Qiagen).
Project description:Proteorhodopsin has been an ongoing hot topic for the past decade. However the complete physiological role of this extremely widely distributed protein remains mysterious. In this study we aim to give an insight to the physiology of a proteorhodopsin-containing sea ice bacteria – Psychroflexus torquis using gel-free label-free proteomic approach for the first time. We also addressed the life strategy that used by this organism to successfully inhabit extreme sea ice environment.
Project description:Although the key role of long-distance trade in the transformation of cuisines worldwide has been well-documented since at least the Roman era, the prehistory of the Eurasian food trade is less visible. In order to shed light on the transformation of Eastern Mediterranean cuisines during the Bronze and Early Iron Age, we analyzed microremains and proteins preserved in the dental calculus of individuals who lived during the 2nd millennium BCE in the Southern Levant. Our results provide clear evidence for the consumption of expected staple foods, such as cereals (Triticeae), sesame (Sesamum) and dates (Phoenix). We additionally report evidence for the consumption of soybean (Glycine), probable banana (Musa), and turmeric (Curcuma), which pushes back the earliest known availability of these foods in the Mediterranean by centuries (turmeric) or even millennia (soybean). We find that, from the early 2nd millennium onwards, at least some people in the Eastern Mediterranean had access to food from distant locations, including South Asia, and such trade goods likely reached the Eastern Mediterranean in the form of oils, dried fruits, and spices. These novel insights force us to rethink the complexity and intensity of Indo-Mediterranean trade during the Bronze Age and also the degree of globalization in early Eastern Mediterranean cuisine.
2020-12-22 | PXD021498 | Pride
Project description:EMG produced TPA metagenomics assembly of the PHOTOHETEROPHY IN A MESOSCALE ANTICYCLONIC EDDY IN THE EASTERN MEDITERRANEAN SEA (Mesoscale_eddy) data set.
Project description:Transcriptional profiling of populations in the clam Ruditapes decussatus determined differentiation in gene-expression along parallel temperature gradients and between races of the Atlantic Ocean and West Mediterranean sea.
