Proteomics

Dataset Information

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Metaproteomics to study metabolic handoffs in the Idas modiolaeformis symbiosis

ABSTRACT: The goal of this study was to identify the key functions of the six main symbionts that are hosted in gills of the marine bivalve, Idas modiolaeformis, which lives at deep-sea hydrocarbon seeps and wood falls in the Eastern Atlantic Ocean and the Mediterranean Sea. These symbionts include the main autotrophic methane- and sulfur-oxidizing lineages (Methyloprofundus, Thioglobus, Thiodubillierella), as well as a Methylophagaceae methylotrophic autotroph, a flavobacterial degrader of complex polysaccharides Urechidicola and a Nitrincolaceae heterotroph that specializes in degradation of nitrogen-rich compounds such as peptides and nucleosides. Four I. modiolaeformis individuals were preserved in RNAlater following retrieval from a brine pool habitat in the Eastern Mediterranean at 1,150 m water depth (32° 13.4' N 34° 10.7' E), using a remotely-operated vehicle. RNAlater was discarded after 24 hours, and the specimens were kept at -80°C until DNA/RNA/protein co-extraction using the AllPrep DNA/RNA/Protein Mini Kit (Cat. No. 80004, Qiagen).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Idas Modiolaeformis Bacteria

TISSUE(S): Gill

SUBMITTER: Simina Vintila  

LAB HEAD: Manuel Kleiner

PROVIDER: PXD040273 | Pride | 2023-07-20

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
IDA_V2.fasta Fasta
M1_140min_1200ng_Run1.msf Msf
M1_140min_1200ng_Run1.pep.xml Pepxml
M1_140min_1200ng_Run1.raw Raw
M2_140min_1200ng_Run1.msf Msf
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Publications

Metabolic handoffs between multiple symbionts may benefit the deep-sea bathymodioline mussels.

Zvi-Kedem Tal T   Vintila Simina S   Kleiner Manuel M   Tchernov Dan D   Rubin-Blum Maxim M  

ISME communications 20230520 1

Bathymodioline mussels rely on thiotrophic and/or methanotrophic chemosynthetic symbionts for nutrition, yet, secondary heterotrophic symbionts are often present and play an unknown role in the fitness of the organism. The bathymodioline Idas mussels that thrive in gas seeps and on sunken wood in the Mediterranean Sea and the Atlantic Ocean, host at least six symbiont lineages that often co-occur. These lineages include the primary symbionts chemosynthetic methane- and sulfur-oxidizing gammaprot  ...[more]

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