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Coelomic P. lividus sea urchin microbiota

ABSTRACT: coelomic P. lividus sea urchin microbiota

PROVIDER: PRJEB27034 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2603640_1.fastq.gz	Fastqsanger.gz
	ERR2603640_2.fastq.gz	Fastqsanger.gz
	ERR2603631_1.fastq.gz	Fastqsanger.gz
	ERR2603631_2.fastq.gz	Fastqsanger.gz
	ERR2603632_1.fastq.gz	Fastqsanger.gz

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Project description:Our aim was to study the immune system of a Mediterranean sea urchin Paracentrotus lividus. Using a label free quantitative mass spectrometry approach we analyzed how bacterial lipopolysaccharide (LPS) is able to modulate the coelomocytes proteome and effect its cellular pathways such as cytoskeleton reorganization, stress and energetic homeostasis.

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Comparative genomics of the sea urchin P. lividus highlights contrasting trends of genome and regulatory evolution in deuterostomes

Project description:Sea urchins are emblematic marine animals with a rich fossil record and represent instrumental models for developmental biology. As echinoderms, sea urchins display several characteristics that set them apart from other deuterostomes such as their highly regulative embryonic development and their unique pentaradial adult body plan. To determine whether these characteristics are linked to particular genomic rearrangement or gene regulatory rewiring, we introduce a chromosome-scale genome assembly for sea urchin Paracentrotus lividus as well as extensive transcriptomic and epigenetic profiling during its embryonic development. We found that sea urchins show opposite modalities of genome evolution as compared to those of vertebrates: they retained ancestral chromosomal linkages that otherwise underwent mixing in vertebrates, while their intrachromosomal gene order has evolved much faster between sea urchin species that split 60 Myr ago than it did in vertebrates. We further assessed the conservation of the cis-regulatory program between sea urchins and chordates and identified conserved modules despite the developmental and body plan differences. We documented regulatory events underlying processes like zygotic genome activation and transition to larval stage in sea urchins. We also identified a burst of gene duplication in the echinoid lineage and showed that some of these expanded genes are involved in organismal novelties, such as Aristotle's lantern, tube feet, or in the specification of lineages through for instance the pmar1 and pop genes. Altogether, our results suggest that gene regulatory networks controlling development can be conserved despite extensive gene order rearrangement.

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Proteomic changes occurring along gonad maturation in the edible sea urchin Paracentrotus lividus

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