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Proteomic changes occurring along gonad maturation in the edible sea urchin Paracentrotus lividus

ABSTRACT: We report the proteomic characterization of gonads from wild P. lividus collected along coastal Sardinia, and describe the changes occurring in gonads according to sex and developmental stage. Gonads in the recovery, pre-mature, mature, and spent stages were analyzed using a shotgun proteomics approach based on filter-aided sample preparation followed by tandem mass spectrometry and label-free differential analysis. A detailed characterization of the proteome changes occurring in gonads of both sexes along maturation was achieved. Significant changes were seen in numerous proteins involved in nutrient accumulation and in gamete biology and maturation. Adding to an improved understanding of the P. lividus reproductive cycle in its natural environment, the results described in this work form the basis for defining novel protein markers and procedures for an easier sexing and staging, and for monitoring sea urchin gonad maturation in aquaculture plants.

INSTRUMENT(S): Q-Tof Ultima

ORGANISM(S): Paracentrotus Lividus (common Sea Urchin)

TISSUE(S): Gonad

SUBMITTER: Stefania Ghisaura

LAB HEAD: Maria Filippa Addis

PROVIDER: PXD004200 | Pride | 2016-06-09

REPOSITORIES: Pride

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Proteomic changes occurring along gonad maturation in the edible sea urchin Paracentrotus lividus.

Ghisaura Stefania S Loi Barbara B Biosa Grazia G Baroli Maura M Pagnozzi Daniela D Roggio Tonina T Uzzau Sergio S Anedda Roberto R Addis Maria Filippa MF

Journal of proteomics 20160602

<h4>Unlabelled</h4>The reproductive stage of Paracentrotus lividus strongly influences product quality that, in turn, impacts significantly on the market price. Large, compact and sweet gonads are preferred, and sensory attributes are positively related to the ratio of nutritive phagocytes to gametes. Gonads at advanced maturation stages, although larger, have less desirable attributes, being more watery and bitter especially in females. Therefore, the best compromise among size, texture, and ta ...[more]

PMID: 27265320

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