Age-dependent epigenetic changes in male germ cells.
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB28044 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Human blood plasma is a complex which communicates with most parts of the body and reflects changes in the state of the organism, therefore identifying age-related biomarkers can help to predict and monitor age-related physiological decline and diseases, as well as to find new treatments for diseases. In this study, TMT-LC-MS/MS was utilized to screen for the differentially expressed plasma proteins in 118 healthy adults with different ages. Participants were divided into three groups: 21-30 (Young), 41-50 (Middle) and ≥60 (Old), the number of differentially expressed proteins when Young vs Middle, Middle vs Old and Young vs Old were 82, 22 and 99, respectively. These proteins were found to be involved in numerous physiological processes such as “negative regulation of smooth muscle cell proliferation” and “blood coagulation”. Meanwhile when Young vs Middle or Old, “Complement and coagulation cascades” was confirmed the top enriched pathway by KEGG pathway enrichment analysis. Functional phenotyping of the proteome demonstrated that the plasma proteomic profiles of Young adults were strikingly dissimilar to the Middle or Old adults. The results of this study mapped the variation in expression of plasma proteins, and provided information about possible biomarkers/treatments for different kinds of age-related function disorders.
2020-10-19 | PXD016199 | Pride
Project description:Male germ cells from young and aged Rats were Isolated and cultured and then treated with pro-oxidant SIN-1 and antioxidant EUK134 Study of the response of isolated male germ cells from young and aged Brown Norway Rats to oxidative stress. Treatment of Isolated and cultured germ cells with pro-oxidant and antioxidant.
2015-04-30 | E-GEOD-66976 | biostudies-arrayexpress
Project description:Male germ cells from young and aged Rats were Isolated and cultured and then treated with pro-oxidant SIN-1 and antioxidant EUK134 Study of the response of isolated male germ cells from young and aged Brown Norway Rats to oxidative stress.
2015-04-30 | GSE66976 | GEO
Project description:We report the transcriptomic profiling of islet cell subtypes obtained from young and old human donors. We report the transcriptomic profiling of Endo-ßH1C cells expressing SIX3, SIX2 or GFP.
2016-04-14 | GSE57973 | GEO
Project description:Muscle biopsies taken from vastus lateralis muscle of 15 men and 15 women after 3 days of standardized diet and activity to examine effects of sex and age Subjects were either young adults (7 men and 7 women, 20-29 yr old) or older (8 men and 8 women, 65-75 yr old) Affymetrix U133A and U133B arrays were scanned both before (S1) and after (S2) antibody enhancement. This file has U133A S1 data. Effects of age were computed for men and women separately with an alternative method previously and data submitted as GSE362 (men) and GSE674 (women). Keywords: sex differences, age-related differences
2007-11-24 | GSE9676 | GEO
Project description:Early life stress (ELS) causes long-lasting changes in gene expression through epigenetic mechanisms. However, little is known about the effects of ELS in adulthood, specifically across different age groups. In this study, the epigenetic modifications of p11 expression in adult mice subjected to ELS were investigated in different stages of adulthood. Pups experienced maternal separation (MS) for 3 h daily from postnatal day 1 to 21. At young and middle adulthood, behavioral test, hippocampal p11 expression levels, and levels of histone acetylation and methylation and DNA methylation at the hippocampal p11 promoter were measured. Middle-aged, but not young adult, MS mice exhibited increased immobility time in the forced swimming test. Concurrent with reduced hippocampal p11 levels, mice in both age groups showed a decrease in histone acetylation (AcH3) and permissive histone methylation (H3K4me3) at the p11 promoter, as well as an increase in repressive histone methylation (H3K27me3). Moreover, our results showed that the expression, AcH3 and H3Kme3 levels of p11 gene in response to MS were reduced with age. DNA methylation analysis of the p11 promoter revealed increased CpG methylation in middle-aged MS mice only. The results highlight the age-dependent deleterious effects of ELS on the epigenetic modifications of p11 transcription.
| S-EPMC8410943 | biostudies-literature
Project description:We use RNA-sequencing to measure global transcription in uteri of young and aged (~1yr old) C57BL/6 females at day 3.5 (E3.5) of pregnancy, and of the decidual part of E11.5 placentas developed in young and aged (~1yr old) females. We report dys-regulation of gene expression in the E3.5 uteri and E11.5 deciduae of aged mice. We compare the transcriptome of young and aged E11.5 deciduae with that of young deciduae from E9.5-E12.5 of pregnancy, and report that aged E11.5 deciduae are developmentally retarded in comparison to young deciduae.
2017-09-14 | GSE98901 | GEO
Project description:Caloric restriction (CR) is considered to increase lifespan and to prevent various age-related diseases in different non-human organisms. Only a limited number of CR studies have been performed in humans, and results put CR as a beneficial tool to decrease risk factors in several age-related diseases. The question remains at what age CR should be implemented to be most effective with respect to healthy aging. The aim of our study was to elucidate the role of age in the transcriptional response to a 30% CR diet in immune cells, as immune response is affected during aging. Ten healthy young men, aged 20-34, and nine healthy old men, aged 64-85, were subjected to a two week weight maintenance diet, followed by three weeks of 30% CR. Before and after 30% CR, peripheral blood mononuclear cells’ (PBMCs) whole genome gene expression was assessed. Expression of 554 genes showed a different response between young and old men upon CR. Gene set enrichment analysis revealed a downregulation of gene sets involved in immune response in young men, but not in old men. At baseline, immune response-related genes were already higher expressed in old compared to young men. Upstream regulator analyses revealed that most potential regulators were controlling immune response, and were inhibited in young men upon CR, and activated in old men at baseline. Based on the gene expression data, we conclude that a short period of CR is more effective in young men compared to old men regarding immune related pathways.
2016-10-24 | GSE63117 | GEO
Project description:We collected the hypothalamus of mice of different ages to ascertain the biomarkers of aging via comparing the transcriptome changes between old and young WT (C57BL/6J) mice.
2023-08-21 | GSE157025 | GEO
Project description:Ovarian somatic cells are essential for tissue function, but there are no ex vivo models which maintain the cellular heterogeneity, in addition to the cell-cell and cell-matrix interactions of this compartment. We engineered a novel ovarian somatic organoid model in which a stroma-enriched fraction of primary mouse ovarian somatic cells was cultured in scaffold-free agarose micromolds. We sought to use this ovarian somatic organoid model to investigate mechanisms of ovarian aging and performed single cell RNA sequencing using primary ovarian somatic cells isolated from reproductively young and old mice, as well as organoids generated from young and old mice at days 1 and 6 of culture to assess age-dependent changes to cellular composition.
2024-08-02 | GSE273891 | GEO