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Age-dependent Response of Isolated Male Germ Cells to Oxidative Stress

ABSTRACT: Male germ cells from young and aged Rats were Isolated and cultured and then treated with pro-oxidant SIN-1 and antioxidant EUK134 Study of the response of isolated male germ cells from young and aged Brown Norway Rats to oxidative stress. Treatment of Isolated and cultured germ cells with pro-oxidant and antioxidant.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Johanna Selvaratnam

PROVIDER: E-GEOD-66976 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Age-dependent Response of Isolated Male Germ Cells to Oxidative Stress

Project description:Male germ cells from young and aged Rats were Isolated and cultured and then treated with pro-oxidant SIN-1 and antioxidant EUK134 Study of the response of isolated male germ cells from young and aged Brown Norway Rats to oxidative stress.

2015-04-30 | GSE66976 | GEO

Expression data of CD9 positive germ cells from young and aged Sprague Dawley germ cell specific GFP rats

Project description:Male fertility and testis function changes with age and so it was sought to determine if these changes are accompanied by changes in gene expression in the spermatogonial cells at the foundation of spermatogenesis A full genome microarray was used to determine if distinct pathways of genes were altered in expression in CD9-positive germ cells with age Testes from young (4 months) and aged (21 months) Sprague Dawley rats were subjected to dissociation to isolate a singel cell suspension of germ cells. These cells were used to isolate CD9 positive spermatogonial cells. RNA was isolated from these cells for hybridization on agilent miroarrays.

2013-12-05 | E-GEOD-50143 | biostudies-arrayexpress

MicroRNA-188 regulates age-related switch between osteoblast and adipocyte differentiation

Project description:To identify microRNAs which differentially expressed in the BMSCs of aged and young mice and and investigate its influences on BMSCs differentiation with ageing. The microRNAs expressions of BMSCs from 3 aged mice and 3 young mice were measured.

2014-04-29 | E-GEOD-57127 | biostudies-arrayexpress

Transcriptional profiling of senescent cell-associated genes in adult mouse lung.

Project description:To evaluate the senescent cell-dependent gene expression in aged lung tissue, young (2mo) ARF-DTR mice and adult (12mo) ARF-DTR mice treated with PBS or DT every two weeks up to 1 month were analyzed using the Agilent Technologies SurePrint G3 Mouse Gene Expression Microarray. Gene expression in lungs of ARF-DTR mice (2mo, 12mo, 12mo+DT) was analyzed. WT mice was used to evaluate the transgene-independent effect of DT on gene expression.

2016-06-27 | E-GEOD-64754 | biostudies-arrayexpress

Aging effect on interfollicular epidermis (paw skin)

Project description:We have employed whole genome microarray expression profiling to identify genes with the potential to distinguish young and aged interfollicular epidermis. Gene expression of paw whole skin samples from 4 young (6 week-old) and 4 aged (102 week-old) C57BL6 mice was analyzed.

2017-07-11 | E-MTAB-4916 | biostudies-arrayexpress

Circadian adaptive signaling to critical ROS stress for cell survival

Project description:Disruption of circadian clocks exacerbates various diseases, in part likely due to impaired stress resistance. It is unclear how nearly lethal stresses affect circadian clocks. We found that near-lethal doses of reactive oxygen species (ROS)-induced critical oxidative stress (cOS) at the branch point of life and death resets circadian clocks, synergistically evoking protective responses for cell survival. The cOS-triggered clock resetting and pro-survival responses are mediated by transcription factor, central clock-regulatory BMAL1 and heat shock stress-responsive (HSR) HSF1. Casein kinase II (CK2) M-bM-^@M-^Smediated phosphorylation regulates dimerization and function of BMAL1 and HSF1 to control the cOS-evoked responses. The core cOS-responsive transcriptome includes CK2-orchestrated crosstalk between the circadian, HSR, NFM-NM-:B-mediated anti-apoptotic, and Nrf2-mediated anti-oxidant pathways. This novel circadian-adaptive signaling system likely plays fundamental protective roles in ROS-inducible disorders, various diseases, and death. Gene expression was measured 4h, 20h and 32h after treatment with 5 mM H2O2 for 10 min.

2013-11-09 | E-GEOD-47955 | biostudies-arrayexpress

Aged gut microbiota contributes to systemical inflammaging after transfer to germ-free mice

Project description:Advanced age is associated with chronic low-grade inflammation, which is usually referred to as inflammaging. Elderly are also known to have an altered gut microbiota composition. However, whether inflammaging is a cause or consequence of an altered gut microbiota composition is not clear. In this study gut microbiota from young or old conventional mice was transferred to young germ-free mice. Four weeks after gut microbiota transfer immune cell populations in spleen, Peyer’s patches, and mesenteric lymph nodes from conventionalized germ-free mice were analyzed by flow cytometry. In addition, whole-genome gene expression in the ileum was analyzed by microarray. Gut microbiota composition of donor and recipient mice was analyzed with 16S rDNA sequencing. Here we show by transferring aged microbiota to young germ-free mice that certain bacterial species within the aged microbiota promote inflammaging. This effect was associated with lower levels of Akkermansia and higher levels of TM7 bacteria and Proteobacteria in the aged microbiota after transfer. The aged microbiota promoted inflammation in the small intestine in the germ-free mice and enhanced leakage of inflammatory bacterial components into the circulation was observed. Moreover, the aged microbiota promoted increased T cell activation in the systemic compartment. In conclusion, these data indicate that the gut microbiota from old mice contributes to inflammaging after transfer to young germ-free mice.

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Gene expression pattern of pulmonary CD11c+ cells from middle-aged and young mice.

Project description:Analysis of function of CD11c+ cells from middle-aged and young mice at gene level. This experiment provided insight into the different genes that plays roles in inflammation, immune response and mainly arachidonic acid cascade that are differentiall expressed in CD11c+ cells from middle aged and young mice. Total RNA was isolated from pulmonary CD11c cells (separated using magnetic beads) from middle-aged and young mice

2015-08-11 | E-GEOD-71868 | biostudies-arrayexpress

Transcriptome analysis of medaka germ cells and gonadal somatic cells during the gonadal sex differentiation.

Project description:Microarray analysis was performed using germ cells and gonadal somatic cells isolated by Fluorescence Activated Cell Sorter (FACS) from XX and XY embryos at st.33 and st.35. Medaka germ cells (olvas-EGFP) and somatic cells (sox9b-DsRed) were isolated by FACS. The microarray experiments were done using three independent biological replicates.

2014-08-31 | E-GEOD-59521 | biostudies-arrayexpress

Crucial genes and pathways in chicken germ stem cell differentiation

Project description:The male germ cell differentiation is a subtle and complex regulation processes, currently its regulatory mechanism is not fully understood. In our experiment, we performed the first comprehensive genome wide analyses of the crucial genes in three kinds of crucial cells (ESCs, PGCs and SSCs) associated with the male germ cells differentiation. We identified thousands of differentially expressed genes (DEGs) in this process andwe choosed 173 candidate genes involved in the differentiation and metabolic processes, like GAL9, AMH, PLK1, PSMD7 and so on. Further exploration found that the candidate genes express patterns were the same between in vitro induction experiments and transcriptome results. Our results clues to the mechanistic basis of male germ cell differentiation and provides an important reference for future studies. Gene expression in chicken germ stem cells was measured at different stages of development. Three independent experiments were performed at each stage (ESCs, PGCs and SSCs).

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