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Chip-seq of BlaER1 B cell transdifferentiation

ABSTRACT: Chip-seq of BlaER1 B cell transdifferentiation

PROVIDER: PRJEB28056 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2722690.fastq.gz	Fastqsanger.gz
	ERR2722691.fastq.gz	Fastqsanger.gz
	ERR2722692.fastq.gz	Fastqsanger.gz
	ERR2722693.fastq.gz	Fastqsanger.gz

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Comparative transcriptomics of B cell transdifferentiation in human and mouse. ChIP-Seq data

Project description:Exogenous overexpression of CEBPA transcription factor induces transdifferentiation from pro B cells into functional macrophages. Here we report the CEBPA binding ChIP-Seq data at 12 hours time point after induction of transdifferentiation in human BLaER1 cells (Rapino et al. 2013).

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Epigenetics profiling of human BLaER1 pre-B cells transdifferentiation into macrophages

Project description:BLaER1 is a human B cell precursor leukemia cell line derived from the RCH-ACV cells. These cells are stably infected with a construct that overexpresses the transcription factor C/EBPa fused with the estrogen receptor hormone binding domain (ER) and GFP. Upon induction with beta-estradiol, C/EBP is internalized into the nucleus, promoting massive transcriptional changes and inducing the transdifferentiation of these pre-B cells into functional macrophages. This process, that lasts 7 days, can be monitored by the detection of specific B cell and macrophage surface markers by flow cytometry. With the goal of understanding the interplay between chromatin and transcription, we have obtained the epigenetic profile of 9 histone modifications (H3K4me1, H3K4me2, H3K4me3, H3K9ac, H3K27ac, H3K36me3, H4K20me2, H3K9me3 and H3K27me3) by ChIP-Seq in twelve time points along the transdifferentiation process, in two biological replicates.

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Epigenetic analysis of BlaER1 transdifferentiation

Project description:Epigenetic analysis of BlaER1 transdifferentiation

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Project description:Study of BLaER1 cell line epigenetic changes induced throughout transdifferentiation. The Illumina Infinium MethylationEPIC Beadchip was used to obtain genomewide methylation profiles of BLaER1 cells at 7 different times throughout transdifferentiation treatment (0h, 3h, 12h, 24h, 48h, 72h and 168h). As a reference, the parental RCH-ACV cell line at 168h of treatment and anonymous donor blood derived macrophages were also profiled.

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Project description:BLaER1 is a human B cell precursor leukemia cell line derived from the RCH-ACV cells. These cells are stably infected with a construct that overexpresses the transcription factor C/EBPa fused with the estrogen receptor hormone binding domain (ER) and GFP. Upon induction with beta-estradiol, C/EBP is internalized into the nucleus, promoting massive transcriptional changes and inducing the transdifferentiation of these pre-B cells into functional macrophages. This process, that lasts 7 days, can be monitored by the detection of specific B cell and macrophage surface markers by flow cytometry. With the goal of understanding the interplay between chromatin and transcription, we have generated transcriptomic data by RNA-Seq in twelve time points along the transdifferentiation process, in two biological replicates.

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Project description:Transdifferentiation of BLaER1 B cell into macrophages is an appropriate model to understand how chromatin behaves along a dynamic process. With this purpose, we have performed chromatin immunoprecipitation experiments of two histone modifications associated to active enhancer activity along 4 time points of BLaER1 transdifferentiation.

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