Project description:U2OS cells expressing DOX inducible pTRIPZ shRNA against IKKbeta or EDC4 were irradiated or left untreated. RNA stability was determined as through actinomycin D chase experiment.
Project description:Goal of the study was to investigate genes regulated differentially after 90 minutes versus 7 days after irradiation. 7 day time point corresponds to cellular senescence. U2OS cells were irradiated with 20 Gy and harvested either 90 minutes or 7 days. In addition cells were left untreated (control) or had knockdown of NFKBIA (protein name Ikappa B alpha). Ikappa B alpha is the main inhibitor of the transcription factor NFkappa B. NFkappa B regulates transcription of many factors constituting the SASP (senescence associated secretory phenotype).
Project description:The goals of this study are to compare the transcriptomes in all cell types from brain microenvironment between irradiated and untreated mouse
Project description:The F-Box-Protein NIPA might define a novel regulator of aging and critical stress response in hematopoietic stem cells To investigate the underlying mechanisms and activated pathways of NIPA-mediated HSC function in steady state and under stress conditions, we performed Affimetrix-based global gene expression analysis on untreated and irradiated, aged Nipa WT and Nipa KO LSK (Lineage-, SCA1+, cKIT+) cells and identified different expression profiles depending on NIPA
Project description:We report the transcriptome profiling (RNAseq) in hepatoblast-derived tumors, without (untreated) or with ST18 knockdown (4h Doxy) (three independent samples for each condition). We find that ST18 knockdown in hepatoblast-derived tumors affects inflammation-associated genes.
Project description:Tumors engender an environment dominated by M2 differentiated tumor macrophages that support tumor invasion, metastases and escape from immune control. In this study, we demonstrate that following radiation therapy of tumors in mice there is an influx of tumor macrophages that polarize towards wound repair and immune suppression. To investigate changes in the phenotype of tumor macrophages following radiation therapy, we FACS sorted tumor macrophages from Panc02 tumors. We have previously shown that we can distinguish mature tumor macrophages from immature myeloid and MDSC populations by expression of Gr1 and IA (MHC class II). To isolate these sub-populations, we first gated CD11b+ cells in the untreated or irradiated tumors, then sorted the CD11b+IA+ macrophage population and the CD11b+Gr1hi MDSC population. Cytospins of the sorted populations demonstrates that the CD11b+Gr1hi MDSC predominantly have a granulocyte morphology and the CD11b+IA+ cells have a macrophage morphology in both the untreated and irradiated tumors. RNA was purified from CD11b+IA+ macrophages from untreated or irradiated tumors 1 day or 7 days following radiation therapy and Gene Expression Microarray analysis was performed.