Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RADseq of T.oceanicus F3 cross

ABSTRACT: SBfI RADseq of Teleogryllus oceanicus F3 cross between Daintree and Kailua flat-wing crickets.

PROVIDER: PRJEB29921 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2990350_1.fastq.gz	Fastqsanger.gz
	ERR2990350_2.fastq.gz	Fastqsanger.gz
	ERR2990360_1.fastq.gz	Fastqsanger.gz
	ERR2990360_2.fastq.gz	Fastqsanger.gz
	ERR2990370_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 1158

Similar Datasets

Teleogryllus oceanicus (field cricket) wing bud proteomics: iTRAQ data

Project description:In Hawaii, a rapidly-evolving mutation in the field cricket Teleogryllus oceanicus silences males by interfering with the development of sound-producing structures on their forewings. The mutation is called flatwing (fw), and it persists because of natural selection imposed by an acoustically-orienting parasitoid. We examined gene expression differences between wild-type and mutant crickets, focusing on juvenile wing buds. We profiled mRNA expression levels using RNA-seq, and characterized the wing bud proteome using quantitative mass spectrometry.

2016-03-31 | PXD002451 | Pride

Teleogryllus oceanicus (field cricket) wing bud proteomics

2016-04-06 | PXD002417 | Pride

Teleogryllus oceanicus

Project description:Teleogryllus oceanicus wing bud transcriptome

| PRJNA283744 | ENA

Immunogenetic and tolerance strategies against a novel parasitoid of wild field crickets

Project description:Among the parasites of insects, endoparasitoids impose a costly challenge to host defenses because they use their host’s body for the development and maturation of their eggs or larvae, and ultimately kill the host. Tachinid flies are highly specialized acoustically-orienting parasitoids that release first instar mobile larvae which burrow into the host’s body to feed. We investigated the possibility that Teleogryllus oceanicus field crickets employ post-infestation strategies to maximize survival when infested with the larvae of the parasitoid fly Ormia ochracea. Using crickets from the Hawaiian island of Kauai, where the parasitoid is present, and crickets from the Cook Islands (Mangaia), where the parasitoid is absent, we evaluated fitness consequences of infestation by comparing feeding behavior, reproductive capacity, and survival of males experimentally infested with O. ochracea larvae. We also evaluated genetic mechanisms underlying host responses by comparing gene expression in crickets infested with fly larvae for different lengths of time with that of uninfested control crickets. We observe some differences in fitness (spermatophore production) and survival (total survival time post-infestation) between populations. However, for both traits significant population effects 1) were not associated with the slope of the response to different numbers of larvae and 2) only emerged from models containing body condition at one but not both time points evaluated. Gene expression patterns also revealed population differences in response to infestation. We did not find evidence for consistent differences in genes associated with immunity or stress response. Taken together, these results suggest that coevolution with the fly does not strongly select for either post-infestation resistance or tolerance of parasitoid larvae in male crickets.

2020-06-02 | GSE151539 | GEO

Teleogryllus oceanicus

Project description:Teleogryllus oceanicus overview

| PRJNA305070 | ENA

RADseq

Project description:RADseq

| PRJNA575770 | ENA

RADseq

Project description:RADseq

| PRJNA575772 | ENA

RADseq for tumour mutation burden estimation and mutation signature analysis.

Project description:The utility of RADseq in an experimental setting is also demonstrated, based on our chasacterisation of an APOBEC mutation signature in an APOBEC3A transfected mouse cell line. 0D5 cells, derived from SSM3 cells, were co-transfected with a mixture containing pcDNA3.1 vectors expressing either APOBEC3A or APOBEC3B (kindly donated by Vincent Caval), pcDNA3.1 construct expressing deaminase null APOBEC3A linked to a uracil deglycosylase construct and a plasmid encoding mutant GFP and WT mCherry that is a reporter for APOBEC mutagenesis. Cells were grown, and gDNA extracted, prior to preparation of RADseq libraries using a PstI- MspI double-digest. Libraries underwent a Pippin Prep to select fragments in the size range of 220-520 bp (genomic sequence plus 148 bp of adapters). Single-end sequencing (1x101bp) was performed on an Illumina NovaSeq6000 utilizing v1.5 chemistry. Reads were aligned to mm10 using bwa mem and variants called using the GATK4 pipeline.

2023-06-30 | GSE234146 | GEO

Teleogryllus oceanicus

Project description:Teleogryllus oceanicus Raw sequence reads

| PRJNA464275 | ENA

Teleogryllus oceanicus

Project description:Teleogryllus oceanicus Raw sequence reads

| PRJNA384097 | ENA