Proteomics

Dataset Information

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Teleogryllus oceanicus (field cricket) wing bud proteomics: iTRAQ data

ABSTRACT: In Hawaii, a rapidly-evolving mutation in the field cricket Teleogryllus oceanicus silences males by interfering with the development of sound-producing structures on their forewings. The mutation is called flatwing (fw), and it persists because of natural selection imposed by an acoustically-orienting parasitoid. We examined gene expression differences between wild-type and mutant crickets, focusing on juvenile wing buds. We profiled mRNA expression levels using RNA-seq, and characterized the wing bud proteome using quantitative mass spectrometry.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Teleogryllus Oceanicus (black Field Cricket)

TISSUE(S): Flight Muscle

SUBMITTER: Catherine Botting  

LAB HEAD: Catherine Helen Botting

PROVIDER: PXD002451 | Pride | 2016-03-31

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
F008784.xml Xml
SoniaiTRAQFr15-18half.mgf Mgf
SoniaiTRAQFr15-18half.wiff Wiff
SoniaiTRAQFr15-18half.wiff.mtd Wiff
SoniaiTRAQFr15-18half.wiff.scan Wiff
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Publications

Rapid evolution and gene expression: a rapidly evolving Mendelian trait that silences field crickets has widespread effects on mRNA and protein expression.

Pascoal S S   Liu X X   Ly T T   Fang Y Y   Rockliffe N N   Paterson S S   Shirran S L SL   Botting C H CH   Bailey N W NW  

Journal of evolutionary biology 20160417 6

A major advance in modern evolutionary biology is the ability to start linking phenotypic evolution in the wild with genomic changes that underlie that evolution. We capitalized on a rapidly evolving Hawaiian population of crickets (Teleogryllus oceanicus) to test hypotheses about the genomic consequences of a recent Mendelian mutation of large effect which disrupts the development of sound-producing structures on male forewings. The resulting silent phenotype, flatwing, persists because of natu  ...[more]

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