DEMONA-Haptophytes
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB31858 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Ammonia-oxidizing archaeal (AOA) amoA diversity and relative abundance in Gulf of Mexico sediments (0-2 cm) were investigated using a functional gene microarray; a two color array with a universal internal standard
2013-03-01 | GSE42286 | GEO
Project description:Diversity and ecology of nanophytoplankton in the Gulf of Gdańsk
| PRJEB23971 | ENA
Project description:Ammonia-oxidizing archaeal (AOA) amoA diversity and relative abundance in Gulf of Mexico sediments (0-2 cm) were investigated using a functional gene microarray; a two color array with a universal internal standard Two color array (cy3 and cy5): the universal standard 20 bp oligo (fluoresced with cy5) is printed to the slide with a 70-mer. Environmental DNA sequences (fluoresced with Cy3) within 15% of the 70-mer will bind to it. Signal is the cy3/cy5. Up to four arrays per sample, with two biological replicates made into two targets, each run on duplicate arrays.
2013-03-01 | E-GEOD-42286 | biostudies-arrayexpress
Project description:Physiological changes in response to environmental cues are not easily documented in pelagic copepods using traditional methods. Molecular biological tools provide new approaches to the investigation of difficult to sample organisms such as oceanic zooplankton. Here, we describe the development of a species-specific microarray for high-throughput studies of the physiological ecology of the North Atlantic copepod Calanus finmarchicus. An EST database was generated for this species using a normalized cDNA library derived from adult and sub-adult individuals from the Gulf of Maine. Sequence data were clustered into contigs and annotated using Blastx. Target transcripts were selected, and unique, 50 base-pair long, oligomer probes were designed and synthesized for 995 genes. Bioinformatic processing using Blast2GO software provided detailed information on gene function. The selected targets include a broad representation of biological processes, cellular components, and molecular functions. The microarray was tested on both experimental and ecological samples, i.e. food abundance and two morphotypes exhibiting distinct lipid stores, respectively. Differentially regulated transcripts were identified for both comparisons. Two comparisons were performed: 1) Lipid-rich (fat) and Lipid-poor (thin) morphotypes 2) Copepods kept under high food and low food experimental conditions
2012-03-23 | E-GEOD-34322 | biostudies-arrayexpress
Project description:Differentially expressed genes may provide insight into the underlying mechanisms of Gulf War Illness involved in neurodegeneration.
2021-07-27 | GSE180786 | GEO
Project description:Contrasting levels of beta-diversity and underlying phylogenetic trends indicate different paths to chemical diversity in highland and lowland willow species
Martin Volf*, Jing Vir Leong, Paola de Lima Ferreira, Tereza Volfova, Petr Kozel, Pavel Matos-Maravi, Elvira Horandl, Natascha D. Wagner, Niko Luntamo, Juha-Pekka Salminen, Simon T. Segar, Brian E. Sedio
*Corresponding author: Martin Volf, volf@entu.cas.cz, tel. +420 387775038, Biology Centre, Czech Academy of Sciences, Institute of Entomology, Branisovska 31, 37005 Ceske Budejovice, Czech Republic
Untargeted metabolomics data was generated for the above publication for 29 species of willows in the Czech Republic and Austria, encompassing an elevation gradient spanning >2500 m (from 175 to 2620 m asl). Extraction and instrumental methods are reported in Volf et al. 2023 Ecology Letters and Sedio et al. 2021 Frontiers in Ecology and Evolution, https://doi.org/10.3389/fevo.2021.679638
2023-04-21 | MSV000091773 | MassIVE
Project description:This pilot study enrolled 9 GWI (Gulf War Illness) cases identified from the Department of Veterans Affairs GWI registry, and 11 sedentary control veterans who had not been deployed to the Persian Gulf and were matched to cases by sex, body mass index (BMI) and age.<br>We exposed GWI patients and matched controls to an exercise challenge to explore differences in immune cell function measured by classic immune assays and gene expression profiling.
2009-02-24 | E-MEXP-2069 | biostudies-arrayexpress
Project description:Combat veterans from the Persian Gulf War have unexplained yet persistent impairment in colonic motility due to combat-related toxic exposures. Central to Gulf War-related toxic exposures was the unmitigated ingestion of Pyridostigmine bromide (PB). We previously developed a Gulf War Illness (GWI) mouse model, where acute PB exposure led to immediate disruptions in colonic motility. Here, we explore mechanisms by which acute enteric neuroinflammation produces peristent impairment in colonic motility. GWI mice were exposed to PB transiently, and allowed to recover with no exposures for 1 month. GWI mice had significantly increased amplitudes of colonic contraction and diminished nerve-stimulated colonic relaxation, compared to naive controls. Immunohistological characterization demonstrated persistent chronic damage in enteric neuronal network integrity, accompanied by a significant imbalance in excitatory and inhibitory motor neuronal populations. Inflammatory CD40+ tissue- resident macrophages were identified with enteric neural stem cells in GWI colons, with an increase in secreted inflammatory cytokines. Unbiased transcriptomic analysis corroborated peristent low grade enteric neuroinflammation, overall resulting in impaired repair and regeneration of neural circuity in GWI. Our learnings can be leveraged to design new regenerative therapies for Gulf War veterans, and broadly impact our understanding of severeal inflammatory disorders of the gut.
2024-09-07 | GSE276269 | GEO
Project description:Microbial Ecology Diversity of Natural Habitats Metagenome
| PRJNA324765 | ENA
Project description:Thaumarchaeota Diversity in the Gulf of Alaska
| PRJNA353048 | ENA