Project description:The initial site of smoking-induced lung disease is the small airway epithelium, which is difficult and time consuming to sample by fiberoptic bronchoscopy. We developed a rapid, office-based procedure to obtain trachea epithelium without conscious sedation from healthy nonsmokers (n=26) and healthy smokers (n=19, 27 ± 15 pack-yr). Gene expression differences [fold-change >1.5, p< 0.01, Benjamini-Hochberg correction] were assessed with Affymetrix microarrays. 1,057 probe sets were differentially expressed in healthy smokers vs nonsmokers, representing >500 genes. Trachea gene expression was compared to an independent group of small airway epithelial samples (n=23 healthy nonsmokers, n=19 healthy smokers, 25 ± 12 pack-yr). The trachea epithelium is more sensitive to smoking, responding with 3-fold more differentially-expressed genes than small airway epithelium. The trachea transcriptome paralleled the small airway epithelium, with 156 of 167 (93%) genes that are significantly up- and down-regulated by smoking in the small airway epithelium showing similar direction and magnitude of response to smoking in the trachea. Trachea epithelium can be obtained without conscious sedation, representing a less invasive surrogate “canary” for smoking-induced changes in the small airway epithelium. This should prove useful in epidemiologic studies correlating gene expression with clinical outcome in assessing smoking-induced lung disease.
Project description:Laser Microdissection (LMD) associated to LC-MS/MS were used to analyze the proteome of neoplastic islands and stroma tumor of Formalin-Fixed and Paraffin Embedded (FFPE) OSCC tissues to identify proteins of prognostic value. Label-free protein quantification and statistical analysis revealed neoplastic islands proteins and from stroma tumor as correlated to clinicopathological data including lymphonode status and recurrence.
2018-09-10 | PXD007232 | Pride
Project description:A METAGENOMIC STUDY OF BANANA NEMATODE ANTAGONISTS IN CANARY ISLANDS
| PRJNA540248 | ENA
Project description:Angiostrongylus cantonensis from Australia, Hawaii, French Polynesia and Canary Islands
