Project description:The availability of a reference genome assembly for Atlantic salmon, Salmo salar, SNP genotyping platforms and low cost sequencing is enhancing the understanding of both life history and production-related traits in this important commercial species. We collected and analysed transcriptomes from selected tissues of Atlantic salmon to inform future functional and comparative genomics studies. Messenger RNA (mRNA) was isolated from brain, pituitary, ovary and liver before Illumina sequencing produced a total of 640 million 150-bp paired-end reads. Following read mapping, feature counting and normalization, cluster analysis identified genes highly expressed in a tissue-specific manner. Functional profiling identified gene clusters describing the unique functions of each tissue. Moreover, highly-expressed transcription factors present in each tissue-specific gene cluster were identified. The data and analysis presented are relevant to the emerging Functional Annotation of All Salmonid Genomes (FAASG) initiative that is seeking to develop a detailed understanding of both salmonid evolution and the genomic elements that drive gene expression and regulation.
Project description:This SuperSeries is composed of the following subset Series: GSE26981: Responses to ectoparasite salmon louse (Lepeophtheirus salmonis) in skin of Atlantic salmon GSE26984: Responses to ectoparasite salmon louse (Lepeophtheirus salmonis) in spleen of Atlantic salmon Refer to individual Series
Project description:The Atlantic salmon (Salmo salar) genome contains 10 chitinase encoding genes, but little is known about the function of these chitinases. Three of the chitinase genes have previously been shown to be expressed in the stomach tissue of Atlantic salmon. In the current study we show that the protein products of these genes, the family 18 glycoside hydrolase (GH18) chitinases, Chia.3, Chia.4 and Chia.7 are secreted into the stomach mucosa and are amongst the most abundant proteins in this matrix.
Project description:This SuperSeries is composed of the following subset Series: GSE19111: Conservation genomics of Atlantic salmon (Year One) GSE19119: Conservation genomics of Atlantic salmon (Year Two) Refer to individual Series
Project description:Atlantic salmon was fed with diets based on five plant protein sources combined with soyabean saponins. Diets with corn gluten, sunflower, rapeseed and horsebean produced minor effects while combination of saponins with pea protein concentrate caused enteritis and major transcriptome changes in the distal intestine.
Project description:Salmon alphavirus (SAV) and Moritella viscosa causing respectively pancreatic disease and winter ulcer are among the most important pathogens threatening Atlantic salmon aquaculture. Fish is protected by vaccination with different rate of success. Here, responses to vaccination were assessed followed with pathogen challenges of vaccinated salmon and saline injected control.
Project description:We report the miRNA profile of extracellular vesicles released from Atlantic salmon head kidney white blood cells that have been cultured for 1 day or 5 days.
Project description:This study investigates sex-biased gene expression between populations of Atlantic and Pacific salmon lice, Lepeophtheirus salmonis. Two Atlantic L. salmonis populations were previously used for an array study (GSE56024) while a third dataset using Pacific L. salmonis was novel. Using all three populations, a consensus-based, meta-analysis approach was used to identify sex-biased and sex-specific genes. Two separate experiments were conducted, one for Atlantic and one for Pacific salmon lice. As the Atlantic data has been previously published for other comparisons (GSE56024), only the Pacific data is uploaded here. Lice from three populations (2 in the Atlantic and 1 in the Pacific) were collected for in vitro bioassay analysis using emamectin benzoate. After 24hrs, lice were collected as per treatment protocol below. Males and females from all populations were compared separately before forming a consensus probe list of sex-biased genes concordantly expressed across all three populations. Please note that each raw data file contains three or four 'block' data and each block data correspond to individual sample raw data. Therefore, each raw data file contains raw data for 3-4 samples (as indicated in the description field).
Project description:We investigate the effect of a functional feed for immunostimulation (peptidoglycan extract from bacterial cell wall with nucleotide formulation) on L. salmonis infection levels on Atlantic salmon Salmo salar, and on host and parasite gene expression profiles. Atlantic salmon smolts (~95 g) were fed a control diet, or a low or high dose immunostimulant diet, and then exposed to L. salmonis copepodids in three subsequent exposures. The transcriptome of salmon lice late in the infection attached to either the low dose diet or control diet hosts were compared using a 38K oligonucleotide microarray.
