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RNA sequencing of neutrophils residing in bone marrow, peripheral blood and lung

ABSTRACT: RNA sequencing of neutrophils residing in bone marrow, peripheral blood and lung

PROVIDER: PRJEB38531 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4179870_1.fastq.gz	Fastqsanger.gz
	ERR4179870_2.fastq.gz	Fastqsanger.gz
	ERR4179862_1.fastq.gz	Fastqsanger.gz
	ERR4179862_2.fastq.gz	Fastqsanger.gz
	ERR4179863_1.fastq.gz	Fastqsanger.gz

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RNA sequencing of neutrophils residing in bone marrow, peripheral blood and lung

Project description:Generation of organ-infiltrating neutrophils occurs in hematopoietic tissues and organs, such as bone marrow and spleen, in response to tumor- and host-derived factors. The de novo expanded neutrophils then egress from hematopoietic sites, circulate through the blood vessels and infiltrate into the organ interstitia and parenchyma. During above trafficking process, neutrophils can undergo phenotypic and functional changes in response to tissue environments. To determine the difference among neutrophils residing in the hematopoietic site—BM, circulating in the blood, and those infiltrating in the metastatic organ, the transcriptional profiles of neutrophils were analyzed by RNA sequencing. 4T1 cells were injected into the fourth mammary fat pads of female syngeneic BALB/cJ mice (8-week-old, n = 3). At day 10 (pre-metastatic stage), the mice were euthanized and then CD45+CD11b+Ly6G<high>Ly6C<med> neutrophils from bone marrow (BM), peripheral blood (PB) and lung were isolated by fluorescence-activated cell sorting. Total RNA was isolated from neutrophils using the miRNeasy Mini kit (Qiagen) and the transcriptional profiles of neutrophils were analyzed by RNA sequencing

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