Project description:High fertility and low fertility bulls were screened from the a 6000 bull database and then identify the sperm-derived DMR and DMC that assoiated with bull ferrtility via whole genome methtlation sequencing
Project description:Pregnancy rates for elite bulls used in artificial insemination (AI) can vary significantly and therefore the identification of molecular markers for bull fertility and targets to improve bull selection is important. β-defensins are peptides with diverse regulatory roles in sperm function across multiple species. To explore the functional impact of DEFB103 CNV on the uterine response in vivo, 18 heifers were inseminated with sperm from bulls categorized by low, intermediate, and high CN levels. Transcriptomic analysis of uterine tissue collected 12 hours after insemination revealed significant differential expression of 58 genes (FDR<0.1) related to sperm migration, immune signaling, and chemotaxis. These findings highlight the significant role of DEFB103 CN in both sperm function and the uterine response to bull sperm, suggesting its potential influence on pregnancy outcomes in cattle.
Project description:This study evaluated the effect of enhanced dietary intake during the early life period on testes transcriptome in bull calves. Between 2-12 weeks of age bull alves were offered either a high (HI; n=15) or moderate (MOD; n=15) plane of nutrition, with diets designed to evoke growth rates of 1.0 and 0.5 kg/day, respectively. At 12 wk of age, testes parenchyma tissue samples were harvested from all calves and subsequently subjected to mRNAseq. Subsequent bioinformatics analyses revealed differential expression of genes invovled in cellular adhesion and immune function.
Project description:Using Illumina® BovineHD Genotyping BeadChip assay, we applied single sperms genotyping from one single Holstein bull to preliminarily describe its recombination map. We received 56 single sperms with qualified genotype information and totally detected 1,526 autosomal crossovers.
Project description:Discovery of genes driving axolotl limb regeneration has been challenging due to limited genomic resources. We assembled 42 RNA-Seq samples totaling approximately 1.3 billion 100 base paired-end reads using Trinity (Grabherr M.G. et al, Nature Biotechnology, 2011; Haas B.J. et al, Nature Protocols, 2013): https://github.com/trinityrnaseq/trinityrnaseq/wiki). We created a transcriptome with complete sequence information for most axolotl genes, identified transcriptional profiles that distinguish blastemas from differentiated limb tissues, and uncovered functional roles for cirbp and kazald1 in limb regeneration.
Project description:The transcriptome of the amphipod Echinogammarus marinus was sequenced after exposure to hypoxia following acclimation to different temperatures using 100BP paired-end Illumina HiSeq sequencing. Amphipods were acclimated to two temperatures, 10 or 20 °C, for one week before individuals were then acutely exposed to normoxic (80% air saturation) or hypoxic conditions (30% air saturation) at 10 °C (n = 5 per experimental treatment).