Project description:PABPN1L participates in the process of post-transcriptional regulation and degradation of maternal mRNA.We collect samples of each period from pabpn1l ko and wt female mice during different stages. Each group contained a total of 30 oocytes or zygotes. In the initial step, we spiked 2 × 106 mRNA-EGFP, transcribed in vitro, into each group.Then, RNA was extracted and detected.Reverse transcription was performed on the samples that met the effective concentration.After quality inspection, build the library.GV oocytes, MII oocytes and zygotes were collected from three different PABPN1L KO or WT mice seperately.
Project description:PABPN1L participates in the process of post-transcriptional regulation and degradation of maternal mRNA.So,we collect samples of each period from pabpn1l ko and wt female mice during different stages.Then, RNA was extracted and detected.Reverse transcription was performed on the samples that met the effective concentration.After quality inspection, build the library.GV oocytes, MII oocytes and zygotes were collected from three different PABPN1L KO or WT mice separately.
Project description:After the library was qualified, the library was pooled according to the effective concentration and the demand of target offline data, and was sequenced by Illumina platform. Since PABPN1L can bind the poly(A) tail, we propose that it participates in the process of post-transcriptional regulation and degradation of maternal mRNA. We investigated the mRNA changes in GV oocytes, MII oocytes, and fertilized eggs using RNA-seq. GV oocytes, MII oocytes and zygotes were collected and mixed from three mice of each genotypes separately.
Project description:We analysed 3~4 repeats of 3 groups of mouse MII oocytes including 2-month-old WT, 2-month-old Tet2-KO, 11-month-old WT to find Tet2 function on female mice fertility.
Project description:We profiled transcriptomes from Cnot6l deadenylase knock-out mouse GV oocytes, MII eggs and 1-cell zygotes in order to analyse its function during the oocyte-to-embryo (OET) transition. Transcriptome of wild-type golden hamster GV oocytes was also profiled.
Project description:Assisted reproductive technology (ART) has become an indispensable tool in fertility treatment. Most ART patients respond normally to controlled ovarian stimulation, however some may benefit from the alternative method of oocyte in vitro maturation (IVM), recognized but still underdeveloped method with limited results. As oocytes undergo the transcriptionally silent phase of meiosis, protein synthesis is an important regulator of oocyte development. Here, we have modelled and compared the clinically relevant IVM simulation with in vivo conditions in mouse oocytes and zygotes.