Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Microgravity-induced senescence mechanisms in human stem cell-derived cardiomyocytes

ABSTRACT: Microgravity-induced senescence mechanisms in human stem cell-derived cardiomyocytes

PROVIDER: PRJEB48855 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR7365790.fastq.gz	Fastqsanger.gz
	ERR7365791.fastq.gz	Fastqsanger.gz
	ERR7365792.fastq.gz	Fastqsanger.gz
	ERR7365793.fastq.gz	Fastqsanger.gz
	ERR7365788.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Microgravity-induced senescence mechanisms in human stem cell-derived cardiomyocytes

Project description:A Comprehensive Study To Decipher The Impact Of Simulated Microgravity On Human Induced Pluripotent Stem Cell-derived Cardiomyocytes

2021-12-05 | E-MTAB-11159 | biostudies-arrayexpress

Microgravity-induced stress mechanisms in human stem cell-derived cardiomyocytes.

Project description:Exposure to outer space microgravity poses a risk for the development of various pathologies including cardiovascular disease. To study this, we derived cardiomyocytes (CMs) from human-induced pluripotent stem cells and exposed them to simulated microgravity (SMG). We combined different "omics" and chromosome conformation capture technologies with live-cell imaging of various transgenic lines to discover that SMG impacts on the contractile velocity and function of CMs via the induction of senescence processes. This is linked to SMG-induced changes of reactive oxygen species (ROS) generation and energy metabolism by mitochondria. Taken together, we uncover a microgravity-controlled axis causing contractile dysfunctions to CMs. Our findings can contribute to the design of preventive and therapeutic strategies against senescence-associated disease.

| S-EPMC9249673 | biostudies-literature

Microgravity on the International Space Station promotes survival and proliferation of human stem cell cardiomyocytes

Project description:Efficient generation of functional cardiomyocytes from human induced pluripotent stem cells (hiPSC-CMs) is critical for their use in regenerative medicine and other applications. In this study, we evaluated the effect of space microgravity (µg) on the differentiation of hiPSC-derived cardiac progenitors compared with parallel 1g condition on the International Space Station. Cryopreserved 3D cardiac progenitors derived from hiPSCs were cultured for 3 weeks. Compared with 1g culture, the µg culture had larger sphere sizes, increased expression of proliferation markers, higher counts of nuclei, and higher cell viability. Highly enriched cardiomyocytes generated in µg had appropriate gene expression and cardiac structure as well as improved function including contractility and Ca2+ handling. RNA-seq analysis of 3-day cultures revealed that short-term exposure of cardiac progenitor spheres to space microgravity upregulated genes involved in cell proliferation, cardiac differentiation, and contraction. These results indicate that space microgravity increased survival and proliferation of hiPSC-CMs and improved their structures and functions.

2022-09-08 | GSE188793 | GEO

Ageing in space: microgravity triggers ferroptosis and accelerates senescence in osteoblastic cells

Project description:To understand the cellular and molecular mechanisms allowing MG63 osteoblastic cells to perceive, transduce and react to microgravity environment.

2023-12-20 | GSE224805 | GEO

microRNA (miRNA) expression data from induced pluripotent stem cell derived (iPSC) derived cardiomyocytes exposed to VX

Project description:Chemical warfare nerve agents (CWNA) are potent cholinesterase inhibitors that may also have non-cholinesterase effects. Several in vivo studies have shown that exposure to CWNA compounds induces damage in the brain and heart. Underlying mechanisms of this damage are a critical area of research for the development of medical countermeasures. This study utilized microRNA (miRNA) analysis to evaluate potential direct cellular effects of the nerve agent VX (o-ethyl-s-[2 (diisopropylamino) ethyl]) on human (iPSC)-derived neurons and iPSC-derived cardiomyocytes. iPSC-derived cardiomyocytes were treated with VX at concentrations of 0µM (saline control), 0.1µM or 100µM for either 1 hour or 6 hours. Total RNA was then isolated and processed for miRNA microarray analysis using Affymetrix miRNA 2.0 GeneChips

2013-12-31 | E-GEOD-48470 | biostudies-arrayexpress

Space microgravity increases expression of genes associated with proliferation and differentiation in human cardiac spheres

Project description:Previously, we reported upregulation of cardiac proliferation in cardiac progenitors derived from human induced pluripotent stem cells (hiPSC-CPCs) that were exposed to space microgravity for 3 days. The overall objective of this investigation was to understand how long-term exposure to microgravity affects the expansion and differentiation of hiPSC-CPCs. Cryopreserved cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CMs) were exposed to space microgravity for 3 weeks in the International Space Station (ISS) and retrieved for transcriptomic profiling. RNA-seq analysis and gene ontology analysis revealed upregulation of cardiac tissue function and morphological terms while downregulating inflammation and ECM regulation terms. Upregulation of genes associated with cell cycle regulation and proliferation were notable as well. Combined with previous work, these results suggest that space microgravity improved cardiac differentiation and structure formation and potentially, proliferation.

2023-12-04 | GSE228063 | GEO

action of microgravity on root development-Action of microgravity on root development

Project description:au11-03_gravite - action of microgravity on root development - Action of microgravity on root development - Arabidopsis were grown on horizontal or vertical clinostat for 4, 8 or 12 days. Seedlings on horizontal clinostat were in simulated microgravity and seedlings on vertical clinostat are considered as a control. Comparison was made between plants grown on simulated microgravitry and vertical position.

2012-08-01 | GSE39631 | GEO

microRNA (miRNA) expression data from induced pluripotent stem cell derived (iPSC) derived cardiomyocytes exposed to soman/GD

Project description:Chemical warfare nerve agents (CWNA) are potent cholinesterase inhibitors that may also have non-cholinesterase effects. Several in vivo studies have shown that exposure to CWNA compounds induces damage in the brain and heart. Underlying mechanisms of this damage are a critical area of research for the development of medical countermeasures. This study utilized microRNA (miRNA) analysis to evaluate potential direct cellular effects of the nerve agent GD/soman (O-Pinacolyl methylphosphonofluoridate) on human-induced pluripotent stem cell (iPSC)-derived cardiomyocytes iPSC-derived cardiomyocytes were treated with GD at concentrations of 0µM (saline control), 0.1µM or 100µM for either 1 hour or 6 hours. Total RNA was then isolated and processed for miRNA microarray analysis using Affymetrix miRNA 2.0 GeneChips

2013-12-31 | E-GEOD-48469 | biostudies-arrayexpress

Melatonin ameliorates simulated-microgravity-induced mitochondrial dysfunction and lipid metabolism dysregulation in hepatocytes

Project description:The liver is an essential multifunctional organ, which constantly communicates with nearly all tissues. It has raised the concern that microgravity exposure can lead to liver dysfunction and metabolic syndromes. However, systematic studies, molecular mechanisms, and intervention measures of the adverse effects of microgravity on hepatocytes are limited. In this study, we utilized the Random Positioning Machine culture system to investigate the adverse effects on hepatocytes under simulated microgravity (SMG). Our results showed that SMG impaired hepatocyte viability, causing cell cycle arrest and apoptosis. Compared to normal gravity (NG), it also triggered lipid accumulation, elevated triglyceride (TG) and ROS levels, and impaired mitochondria function in hepatocytes. Furthermore, RNA-seq results showed that SMG upregulated genes implicated in lipid metabolisms, including PPARγ, PLIN2, CD36, FABPs, etc.. Importantly, all these defects can be suppressed by melatonin, a potent antioxidant secreted by the pineal gland, suggesting its potential use as a novel strategy of therapeutic intervention.

2023-04-04 | GSE228604 | GEO

Length-independent telomere damage drives cardiomyocyte senescence

Project description:Ageing is the biggest risk factor to cardiovascular health and is associated with increased incidence of cardiovascular disease. Cellular senescence, a process driven in part by telomere shortening has been implicated in age-related cardiac dysfunction. However, the role of cellular senescence and its underlying mechanisms in slowly dividing/post-mitotic cardiomyocytes is not understood.

2019-02-06 | GSE95822 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data