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Whole-genome sequencing reveals recent transmission of mul-tidrug-resistant Mycobacterium tuberculosis CAS1-Kili strains in Lusaka, Zambia

ABSTRACT: An investigation of recent transmission of multidrug-resistant Mycobacterium tuberculosis CAS1-Kili strains in Lusaka, Zambia

PROVIDER: PRJEB49247 | ENA |

REPOSITORIES: ENA

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Project description:Whole-genome sequencing reveals recent transmission of multidrug-resistant Mycobacterium tuberculosis CAS1-Kili strains in Lusaka, Zambia

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Whole-Genome Sequencing Reveals Recent Transmission of Multidrug-Resistant <i>Mycobacterium tuberculosis</i> CAS1-Kili Strains in Lusaka, Zambia.

Project description:Globally, tuberculosis (TB) is a major cause of death due to antimicrobial resistance. Mycobacterium tuberculosis CAS1-Kili strains that belong to lineage 3 (Central Asian Strain, CAS) were previously implicated in the spread of multidrug-resistant (MDR)-TB in Lusaka, Zambia. Thus, we investigated recent transmission of those strains by whole-genome sequencing (WGS) with Illumina MiSeq platform. Twelve MDR CAS1-Kili isolates clustered by traditional methods (MIRU-VNTR and spoligotyping) were used. A total of 92% (11/12) of isolates belonged to a cluster (≤12 SNPs) while 50% (6/12) were involved in recent transmission events, as they differed by ≤5 SNPs. All the isolates had KatG Ser315Thr (isoniazid resistance), EmbB Met306 substitutions (ethambutol resistance) and several kinds of rpoB mutations (rifampicin resistance). WGS also revealed compensatory mutations including a novel deletion in embA regulatory region (-35A > del). Several strains shared the same combinations of drug-resistance-associated mutations indicating transmission of MDR strains. Zambian strains belonged to the same clade as Tanzanian, Malawian and European strains, although most of those were pan-drug-susceptible. Hence, complimentary use of WGS to traditional epidemiological methods provides an in-depth insight on transmission and drug resistance patterns which can guide targeted control measures to stop the spread of MDR-TB.

| S-EPMC8773284 | biostudies-literature

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