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Microbial profiling of dental plaque from mechanically ventilated patients

ABSTRACT: Microbial profiling of dental plaque

PROVIDER: PRJEB9696 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR928314_1.fastq.gz	Fastqsanger.gz
	ERR928314_2.fastq.gz	Fastqsanger.gz
	ERR928315_1.fastq.gz	Fastqsanger.gz
	ERR928315_2.fastq.gz	Fastqsanger.gz
	ERR928316_1.fastq.gz	Fastqsanger.gz

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Microbial profiling of dental plaque from mechanically ventilated patients.

Project description:Micro-organisms isolated from the oral cavity may translocate to the lower airways during mechanical ventilation (MV) leading to ventilator-associated pneumonia (VAP). Changes within the dental plaque microbiome during MV have been documented previously, primarily using culture-based techniques. The aim of this study was to use community profiling by high throughput sequencing to comprehensively analyse suggested microbial changes within dental plaque during MV. Bacterial 16S rDNA gene sequences were obtained from 38 samples of dental plaque sampled from 13 mechanically ventilated patients and sequenced using the Illumina platform. Sequences were processed using Mothur, applying a 97% gene similarity cut-off for bacterial species level identifications. A significant 'microbial shift' occurred in the microbial community of dental plaque during MV for nine out of 13 patients. Following extubation, or removal of the endotracheal tube that facilitates ventilation, sampling revealed a decrease in the relative abundance of potential respiratory pathogens and a compositional change towards a more predominantly (in terms of abundance) oral microbiota including Prevotella spp., and streptococci. The results highlight the need to better understand microbial shifts in the oral microbiome in the development of strategies to reduce VAP, and may have implications for the development of other forms of pneumonia such as community-acquired infection.

| S-EPMC5115166 | biostudies-literature

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Dental Plaque

Project description:Dental Plaque Targeted loci environmental

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Dental Plaque Microbiome

Project description:Dental Plaque Microbiome Targeted loci environmental

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Transcriptome Analysis of Human Supragingival Dental Plaque Microbiome from 19 Twin Pairs with and without Dental Caries

Project description:The study aims to assess gene expression in plaque samples collected from twin pairs that are both concordant and discordant with respect to dental Caries diagnosis. File Naming Conventions are as follows: Patient ID : 4 digit identifier Diagnosis : Caries Negative(CN) or Caries Positive(CP) Type of Twin: Monozygotic(MZ)or Dizygotic(DZ) Pair to xxxx: 4 digit twin identifier maps to the Patient ID E.g: 2126_CP_MZ_PairTo_2125_fastqc - 2126 is a caries positive patient and pairs to monozygotic twin pair 2125. Plaque samples from twin pairs that are both concordant and discordant with respect to dental Caries diagnosis are enriched for bacterial messenger RNA to study the gene expression differences in the samples. RNA was extracted from RNAprotect (Qiagen, In c.) treated dental plaque scrapings from 38 patients. Amplified cDNA was created and rRNA sequence was removed by subtractive hybridization. Individual patient samples were run on a single lane of an Illumina Genome Analyzer.

2011-09-26 | E-GEOD-31439 | biostudies-arrayexpress

GNPS - Multi-omics of dental plaque in patients with diabetes and periodontal disease

Project description:This repository contains raw files and intermediate processing files for an integrated multi-omics analysis of dental plaque.

2019-07-23 | MSV000084126 | GNPS

Multi-omics of dental plaque in patients with diabetes and periodontal disease

Project description:This repository contains raw files and intermediate processing files for an integrated multi-omics analysis of dental plaque.

2019-07-23 | MSV000084126 | MassIVE

Transcriptome Analysis of Human Supragingival Dental Plaque Microbiome from 19 Twin Pairs with and without Dental Caries

2011-09-26 | GSE31439 | GEO

Native Indian Dental Plaque metagenome

Project description:Species Level Characterization of Dental Plaque Associated Microbiome of Native Indian Children using Metagenome Sequencing

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Distinct signatures of dental plaque metabolic byproducts dictated by periodontal inflammatory status

Project description:Onset of chronic periodontitis is associated with an aberrant polymicrobial community, termed dysbiosis. Findings of a recent model of its etiology suggested that dysbiosis holds a conserved metabolic signature as an emergent property. The purpose of this study was to identify robust biomarkers for periodontal inflammation severity. Furthermore, we investigated disease-associated metabolic signatures of periodontal microbiota using a salivary metabolomics approach. Collection of whole saliva samples was performed before and after removal of supragingival plaque (debridement). Periodontal inflamed surface area (PISA) was employed as an indicator of periodontal inflammatory status. Based on multivariate analyses using pre-debridement salivary metabolomics data, we found that the metabolites associated with higher PISA included cadaverine and hydrocinnamate, while uric acid and ethanolamine were associated with lower PISA. Next, we focused on dental plaque metabolic byproducts by selecting significantly decreased salivary metabolites following debridement. Metabolite set enrichment analysis revealed that polyamine metabolism, arginine and proline metabolism, butyric acid metabolism, and lysine degradation were distinctive metabolic signatures of dental plaque in the high PISA group, which may have relevance to the metabolic signatures of disease-associated communities. Collectively, our findings identified potential biomarkers of periodontal inflammatory status, while they also provide insight into metabolic signatures of dysbiotic communities.

2016-10-23 | ST000496 | MetabolomicsWorkbench

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