Project description:Osteoporosis is a major problem for the laying hen industry due to welfare issues and economic losses related to bone fractures. The objective of this research was to better understand genetic influences on bone integrity by comparing gene expression profiles from bone marrow of chickens expressing phenotypic differences for a number of bone characteristics. Global expression profiles of genes included on the Fred Hutchinson Cancer Research Center (FHCRC) Chicken 13K array were assessed in broiler and layer chickens. Keywords: Gene expression comparison between two chicken lines at 60 weeks of age
2007-01-01 | GSE6270 | GEO
Project description:Differential gene expression in the bone marrow of layer and broiler chickens
Project description:Osteoporosis is a major problem for the laying hen industry due to welfare issues and economic losses related to bone fractures. The objective of this research was to better understand genetic influences on bone integrity by comparing gene expression profiles from bone marrow of chickens expressing phenotypic differences for a number of bone characteristics. Global expression profiles of genes included on the Fred Hutchinson Cancer Research Center (FHCRC) Chicken 13K array were assessed in broiler and layer chickens. Keywords: Gene expression comparison between two chicken lines at 15 weeks of age
Project description:RNA sequencing analysis of abdominal adipose tissue in 7 week old juvenile broiler chickens divergently selected for abdominal fatness or leanness
Project description:RNA sequencing analysis of abdominal adipose tissue in 7 week old juvenile broiler chickens divergently selected for high or low body weight
Project description:Optimization of broiler chicken breast muscle protein accretion is key for the efficient production of poultry meat, whose demand is steadily increasing. In a context where antimicrobial growth promoters use is being restricted, it is important to find alternatives as well as to characterize the effect of immunological stress on broiler chicken growth. Despite of its importance, research on broiler chicken muscle protein dynamics has been mostly limited to the study of mixed protein turnover. The present study aims to characterize the effect of a bacterial challenge and the feed supplementation of a citrus and a cucumber extract on broiler chicken individual breast muscle proteins fractional synthesis rates (FSR) using a recently developed dynamic proteomics pipeline. 21 day-old broiler chickens were administered a single 2H2O dose before being culled at different timepoints. A total of 60 breast muscle protein extracts from five experimental groups (Unchallenged, Challenged, Control Diet, Diet 1 and Diet 2) were analyzed using a DDA proteomics approach. Proteomics data was filtered in order to reliably calculate multiple proteins FSR making use of a newly developed bioinformatics pipeline. Broiler breast muscle proteins FSR uniformly decreased following a bacterial challenge, this change was judged significant for 15 individual proteins, the two major functional clusters identified as well as for mixed breast muscle protein. Citrus or cucumber extract feed supplementation did not show any effect on the breast muscle protein FSR of immunologically challenged broilers. The present study has identified potential predictive markers of breast muscle growth and provided new information on broiler chicken breast muscle protein turnover which could be essential for improving the efficiency of broiler chicken meat production.