Dataset Information

Effect of an immune challenge and feed supplements on broiler chicken individual breast muscle protein synthesis

ABSTRACT: Optimization of broiler chicken breast muscle protein accretion is key for the efficient production of poultry meat, whose demand is steadily increasing. In a context where antimicrobial growth promoters use is being restricted, it is important to find alternatives as well as to characterize the effect of immunological stress on broiler chicken growth. Despite of its importance, research on broiler chicken muscle protein dynamics has been mostly limited to the study of mixed protein turnover. The present study aims to characterize the effect of a bacterial challenge and the feed supplementation of a citrus and a cucumber extract on broiler chicken individual breast muscle proteins fractional synthesis rates (FSR) using a recently developed dynamic proteomics pipeline. 21 day-old broiler chickens were administered a single 2H2O dose before being culled at different timepoints. A total of 60 breast muscle protein extracts from five experimental groups (Unchallenged, Challenged, Control Diet, Diet 1 and Diet 2) were analyzed using a DDA proteomics approach. Proteomics data was filtered in order to reliably calculate multiple proteins FSR making use of a newly developed bioinformatics pipeline. Broiler breast muscle proteins FSR uniformly decreased following a bacterial challenge, this change was judged significant for 15 individual proteins, the two major functional clusters identified as well as for mixed breast muscle protein. Citrus or cucumber extract feed supplementation did not show any effect on the breast muscle protein FSR of immunologically challenged broilers. The present study has identified potential predictive markers of breast muscle growth and provided new information on broiler chicken breast muscle protein turnover which could be essential for improving the efficiency of broiler chicken meat production.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Muscle

SUBMITTER: Eduardo Zarzuela

LAB HEAD: Javier Muñoz

PROVIDER: PXD044325 | Pride | 2024-04-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20210709_EZ_EPIC_Chick_1.raw	Raw
	20210709_EZ_EPIC_Chick_10.raw	Raw
	20210709_EZ_EPIC_Chick_11.raw	Raw
	20210709_EZ_EPIC_Chick_12.raw	Raw
	20210709_EZ_EPIC_Chick_13.raw	Raw

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Effect of an immune challenge and two feed supplements on broiler chicken individual breast muscle protein synthesis rate.

Peinado-Izaguerri Jorge J Corbishley Alexander A Zarzuela Eduardo E Pina-Beltrán Blanca B Riva Francesca F McKeegan Dorothy E F DEF Bain Maureen M Muñoz Javier J Bhide Mangesh M McLaughlin Mark M Preston Tom T

Journal of proteomics 20240313

Optimization of broiler chicken breast muscle protein accretion is key for the efficient production of poultry meat, whose demand is steadily increasing. In a context where antimicrobial growth promoters use is being restricted, it is important to find alternatives as well as to characterize the effect of immunological stress on broiler chicken's growth. Despite its importance, research on broiler chicken muscle protein dynamics has mostly been limited to the study of mixed protein turnover. The ...[more]

PMID: 38484873

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