Project description:Recent advances in high throughput sequencing methodologies allow the opportunity to probe in depth the transcriptomes of organisms including N. caninum. In this project, we are using Illumina sequencing technology to analyze the transcriptome (RNA-Seq) of experimentally accessible stages (e.g. tachyzoites at different times points) of N. caninum NCLiv. The aim is to make transcriptional landscape maps at different time points at different life cycle stages of N. caninum and compare it with equivalent datasets from the closely related parasite Toxoplasma gondii
Project description:Recent advances in high throughput sequencing methodologies allow the opportunity to probe in depth the transcriptomes of organisms including N. caninum. In this project, we are using Illumina sequencing technology to analyze the transcriptome (RNA-Seq) of experimentally accessible stages (e.g. tachyzoites at different times points) of N. caninum NCLiv. The aim is to make transcriptional landscape maps at different time points at different life cycle stages of N. caninum and compare it with equivalent datasets from the closely related parasite Toxoplasma gondii. ArrayExpress Release Date: 2011-02-08 Person Roles: submitter Person Last Name: Service Person First Name: Submission Person Mid Initials: Person Email: datahose@sanger.ac.uk Person Phone: Person Address: The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, United Kingdom Person Affiliation: Wellcome Trust Sanger Institute Person Roles: Investigator Person Last Name: Reid Person First Name: Adam Person Mid Initials: Person Email: ar11@sanger.ac.uk Person Phone: 01223 834244 Person Address: Wellcome Trust Genome Campus,Hinxton,Cambridge. CB10 1SA UK Person Affiliation: Wellcome Trust Sanger Institute Person Roles: Project Coordinator Person Last Name: Sanders Person First Name: Mandy Person Mid Initials: J Person Email: mjs@sanger.ac.uk Person Phone: 01223 834244 Person Address: Wellcome Trust Genome Campus,Hinxton,Cambridge. CB10 1SA UK Person Affiliation: Wellcome Trust Sanger Institute
Project description:Enteric protozoan parasite Entamoeba histolytica is a causative agent of amebiasis. One of the major virulence factors of this parasite is cysteine protease (CP). To understand the molecular basis of intracellular trafficking of CP, we identified CP5 binding protein and named cysteine protease binding protein family 1 (CPBF1). To study the function of CPBF1, we generate CPBF1 gene silenced strain and analyzed the expression profile by custom made microarray.
Project description:Enteric protozoan parasite Entamoeba histolytica is a causative agent of amebiasis. One of the major virulence factors of this parasite is cysteine protease (CP). To understand the molecular basis of intracellular trafficking of CP, we identified CP5 binding protein and named cysteine protease binding protein family 1 (CPBF1). To study the function of CPBF1, we generate CPBF1 gene silenced strain and analyzed the expression profile by custom made microarray. E. histolytica G3 strain was transfected with pSAP2 vector or pSAP2-CPBF1 and established the transformats by selecting the transfected cells with G418. After confirm the gene silencing of CPBF1 by RT-PCR, we performed the transcriptome analysis of pSAP2 (mock) and pSAP2-CPBF1 (gene silenced) cells.
Project description:Transcriptional responses occurring in the spleen in response to N. caninum infection compared to uninfected mice Two conditions: infected and un-infected mice; Biological replicates: three (or four) infected and three uninfected mice. One control and one infected per array