Project description:Manuka honey has been shown to inhibit growth in EMRSA-15 by inhibiting cell division, the mode of actin is currently unclear. We used microarrays to detail the difference in global gene expresson following treatment of cells with honey. We identified distinct classes of up and down regulated genes
Project description:Manuka honey has been shown to inhibit growth in EMRSA-15 by inhibiting cell division, the mode of actin is currently unclear. We used microarrays to detail the difference in global gene expresson following treatment of cells with honey. We identified distinct classes of up and down regulated genes EMRSA-15 were harvested 4 hours after exposure to treatment for RNA extraction and hybridization on Affymetrix microarrays. Control and honey treated cells were investigates, three biological replicates were used for each sample.
Project description:The aim of this study was to characterise the transcriptomic changes in P. aeruginosa PA14 following treatment with antimicrobial manuka honey and its key components (sugar, methyglyoxal, sugar + methylglyoxal) for 30 minutes at a subinhibitory concentration (0.5x MIC). mRNA profiling was conducted to gain mechanistic insights into the mode of action of antimicrobial manuka honey.
Project description:Proteomic analysis was performed on both manuka (Leptospermum scoparium) and non-manuka honeys and nectars to identify unique peptide markers which can be used for manuka honey authentication.
Project description:Manuka honey has been shown to inhibit growth in Pseudomonas aueruginosa, the mode of action is currently unclear. We used microarrays to detail the difference in global gene expresson following treatment of cells with honey. We identified distinct classe of up and down regulated genes
Project description:Manuka honey has been shown to inhibit growth in Pseudomonas aueruginosa, the mode of action is currently unclear. We used microarrays to detail the difference in global gene expresson following treatment of cells with honey. We identified distinct classe of up and down regulated genes Pseudomonas aueruginosa were harvested 4 hours after exposure to treatment for RNA extraction and hybridization on Affymetrix microarrays. Control and honey treated cells were investigates, three biological replicates were used for each sample.
Project description:Young adult fer-15;fem-1 Caenorhabditis elegans were infected with Staphylococcus aureus for 8 h to determine the transcriptional host response to Staphylococcus aureus. Analysis of differential gene expression in C. elegans young adults exposed to two different bacteria: E. coli strain OP50 (control), wild-type Staphylococcus aureus RN6390. Samples were analyzed at 8 hours after exposure to the different bacteria. These studies identified C. elegans genes induced by pathogen infection. Keywords: response to pathogen infection, innate immunity, host-pathogen interactions
