Saccharomyces cerevisiae
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: E-GEOD-37003
PROVIDER: PRJNA157291 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:This SuperSeries is composed of the following subset Series: GSE36958: Gene expression profiles of WT and ime4-/- mutant yeast cells, under vegetative and meiosis-inducing conditions GSE37001: METTL3 KD in HepG2 cells GSE37002: m6A mapping in human RNA (with treatments) GSE37003: m6A mapping in human RNA (untreated) GSE37004: m6A mapping in mouse RNA (mouse liver and human brain) Refer to individual Series
2012-04-24 | E-GEOD-37005 | biostudies-arrayexpress
Project description:Inactivation of the yeast IME4 gene, the yeast homologue of METTL3, was shown to result in the loss of m6A in mRNA of mutant cells grown in sporulation medium. We attempted to characterize the effects of ime4 deletion on gene expression under vegetative and meiosis-inducing conditions. The results show that in vegetatively-growing ime4-/- cells there is an increased expression of the RME1 gene (repressor of meiosis) which prevents precocious entry into the meiotic program. Mutant yeast cells showed reduced expression levels of genes involved in ribosome biogenesis and gene expression processes. Surprisingly, despite the fact that a diploid strain was analyzed, there was also a striking change in the expression level of haploid-specific genes, suggesting that RNA methylation may be used to enforce the sexual identity of diploid cells, required for the implementation of the gametogenesis program. Consistently, when cells were induced to undergo meiosis, ime4-/- diploids failed to undergo the meiotic divisions. Among the genes showing reduced expression in the mutant were IME1 and IME2, the two known inducers of meiosis. Thus, the yeast IME4 gene plays an important role in the regulation of the developmental switch from vegetative cells into gametogenesis. WT and ime4 -/- yeast cells were grown vegetatively in YPD (Yeast extract, Peptone, Dextrose) medium, and transferred to sporulation medium(1% Kacetate) for induction of sporulation for 4 hrs. RNA was purified from the cells and hybridized to Affymetrix microarrays. Experiments were conducted in biological replicates.
2012-04-24 | E-GEOD-36958 | biostudies-arrayexpress
Project description:Inactivation of the yeast IME4 gene, the yeast homologue of METTL3, was shown to result in the loss of m6A in mRNA of mutant cells grown in sporulation medium. We attempted to characterize the effects of ime4 deletion on gene expression under vegetative and meiosis-inducing conditions. The results show that in vegetatively-growing ime4-/- cells there is an increased expression of the RME1 gene (repressor of meiosis) which prevents precocious entry into the meiotic program. Mutant yeast cells showed reduced expression levels of genes involved in ribosome biogenesis and gene expression processes. Surprisingly, despite the fact that a diploid strain was analyzed, there was also a striking change in the expression level of haploid-specific genes, suggesting that RNA methylation may be used to enforce the sexual identity of diploid cells, required for the implementation of the gametogenesis program. Consistently, when cells were induced to undergo meiosis, ime4-/- diploids failed to undergo the meiotic divisions. Among the genes showing reduced expression in the mutant were IME1 and IME2, the two known inducers of meiosis. Thus, the yeast IME4 gene plays an important role in the regulation of the developmental switch from vegetative cells into gametogenesis.
2012-04-25 | GSE36958 | GEO
Project description:Identify which stages of meiosis are impaired in replicative aging cells Transcriptional profile of aging yeast cells (young and old) incubated in sporulation-inducing conditions compared to new-born cells grown in vegetative conditions. Two conditions experiments. Young and Old cells incubated in sporulation-inducing conditions. Control strain was same strain used for the experimental samples but grown in vegetative conditions.
2010-05-08 | E-GEOD-15570 | biostudies-arrayexpress
Project description:Identify which stages of meiosis are impaired in replicative aging cells Transcriptional profile of aging yeast cells (young and old) incubated in sporulation-inducing conditions compared to new-born cells grown in vegetative conditions.
2009-06-16 | GSE15570 | GEO
Project description:WT vs rim101 mutant gene expression under capsule inducing conditions
| PRJNA130327 | ENA
Project description:WT vs gcn5 mutant gene expression under capsule inducing conditions
| PRJNA130413 | ENA
Project description:Transcriptional profiling of C. lusitaniae a/alpha WT cells on 0.37% PDA (potato dextrose agar) for 30 minutes (m), 2 hours (h), 4h, 8h, 12h, 18h, 24, and 30h hybridized against WT a/alpha cells in non-meiosis inducing conditions YPD (yeast peptone dextrose) for 2h
2014-02-01 | GSE51793 | GEO
Project description:Transcriptional profiling of C. lusitaniae a/alpha WT cells on 0.37% PDA (potato dextrose agar) for 30 minutes (m), 2 hours (h), 4h, 8h, 12h, 18h, 24, and 30h hybridized against WT a/alpha cells in non-meiosis inducing conditions YPD (yeast peptone dextrose) for 2h Two condition experiment: a/alpha WT on PDA vs. a/alpha WT on YPD, 4 biological replicates
2014-02-01 | E-GEOD-51793 | biostudies-arrayexpress
Project description:During meiosis in yeast, global splicing efficiency increases. The mechanism for this is relief of competition for the splicing machinery by repression of intron-containing ribosomal protein genes (RPGs). Repression of RPGs with rapamycin also increases splicing efficiency in vegetative cells. Reducing levels of an RPG-dedicated transcription factor globally improves splicing and suppresses the temperature-sensitive growth defect of a spliceosome mutation. These results indicate that the spliceosome is limiting and pre-mRNAs compete with each other. Under these conditions, splicing efficiency of a given pre-mRNA therefore depends on both its concentration and affinity for the limiting splicing factor(s) as well as those of the competing pre-mRNAs. We propose that trans-competition control of splicing helps repress meiotic gene expression in vegetative cells, and promotes efficient meiosis. Competition between RNAs for a limiting factor may be a general condition important for function of a variety of post-transcriptional control mechanisms. Splicing and gene expression profiles of 1) wild type yeast cells treated with rapamycin (2 biological replicates) relative to untreated cells and 2) prp4-1 pGAL-IFH1 (down-regulated expression of IFH1 transcription factor(specific for ribosomal protein genes)) relative to prp4-1 yeast.
2013-10-22 | E-GEOD-44219 | biostudies-arrayexpress