Project description:Anastrepha ludens (Loew) is one of the most important pestiferous fruit fly species infesting citrus and mangoes from Texas to Costa Rica. Despite the latter, the mechanisms of odorant perception in this specie have been poorly studied. In most of the cases, the detection of semiochemicals in insects is carried out in the antenna, where volatile molecules penetrate sensillum pores and they are linked to soluble proteins to cross the lymph until reaching specific odor receptor triggering the signal transduction which can lead to a behavioral response. Scrutinizing the molecular foundation of odorant perception in A. ludens is of paramount importance to increase the efficiency of Integrated Management strategies against this pest. We carried out a proteomic profiling of A. ludens’s antennas with three different maturity stages using Nano-LC-MS/MS. We identified 4618 unique proteins in the antennas of A. ludens across maturity stages. The core proteome of 1697 IDs featured proteins associated with odor perception including odorant-binding proteins (OBP), calcium signaling related proteins, the universal odorant co-receptor Orco and putative odorant-degrading enzymes (ODE).

2021-09-09 | PXD019960 | Pride

Anastrepha ludens strain:Family 10

Project description:Anastrepha ludens strain:Family 10 Raw sequence reads

| PRJNA503495 | ENA

Anastrepha ludens antenna proteome in response to CeraTrap®

Project description:Anastrepha ludens (Loew) is one of the most important pestiferous fruit fly species infesting citrus and mangoes from Texas to Costa Rica. Despite the latter, the mechanisms of odorant perception in this specie have been poorly studied. In most of the cases, the detection of semiochemicals in insects is carried out in the antenna, where volatile molecules penetrate sensillum pores and they are linked to soluble proteins to cross the lymph until reaching specific odor receptor triggering the signal transduction which can lead to a behavioral response. Scrutinizing the molecular foundation of odorant perception in A. ludens is of paramount importance to increase the efficiency of Integrated Management strategies against this pest. Therefore, we carried out a proteomic profiling of A. ludens’s antennas with three different maturity stages using comparative proteomic analysis with tandem mass tags (TMT) combined with synchronous precursor selection (SPS)-MS3 to gain insight into the proteome of the A. ludens antenna under CeraTrap exposure, a highly effective commercial attractant. Our study provides the first report where critical molecular players might form part of the odor perception mechanism in A. ludens.

2021-09-09 | PXD020012 | Pride

Anastrepha ludens breed:Mexican fruit fly

Project description:Anastrepha ludens breed:Mexican fruit fly Transcriptome or Gene expression

| PRJNA555704 | ENA