Dataset Information

Anastrepha ludens antenna proteome

ABSTRACT: Anastrepha ludens (Loew) is one of the most important pestiferous fruit fly species infesting citrus and mangoes from Texas to Costa Rica. Despite the latter, the mechanisms of odorant perception in this specie have been poorly studied. In most of the cases, the detection of semiochemicals in insects is carried out in the antenna, where volatile molecules penetrate sensillum pores and they are linked to soluble proteins to cross the lymph until reaching specific odor receptor triggering the signal transduction which can lead to a behavioral response. Scrutinizing the molecular foundation of odorant perception in A. ludens is of paramount importance to increase the efficiency of Integrated Management strategies against this pest. We carried out a proteomic profiling of A. ludens’s antennas with three different maturity stages using Nano-LC-MS/MS. We identified 4618 unique proteins in the antennas of A. ludens across maturity stages. The core proteome of 1697 IDs featured proteins associated with odor perception including odorant-binding proteins (OBP), calcium signaling related proteins, the universal odorant co-receptor Orco and putative odorant-degrading enzymes (ODE).

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Insecta Anastrepha Ludens

TISSUE(S): Antenna

SUBMITTER: Eliel Ruiz-May

LAB HEAD: Proteomic laboratory

PROVIDER: PXD019960 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

A First Glimpse of the Mexican Fruit Fly Anastrepha ludens (Diptera: Tephritidae) Antenna Morphology and Proteome in Response to a Proteinaceous Attractant.

Ruiz-May Eliel E Altúzar-Molina Alma A Elizalde-Contreras José M JM Arellano-de Los Santos Jiovanny J Monribot-Villanueva Juan J Guillén Larissa L Vázquez-Rosas-Landa Mirna M Ibarra-Laclette Enrique E Ramírez-Vázquez Mónica M Ortega Rafael R Aluja Martín M

International journal of molecular sciences 20201029 21

Anastrepha ludens is a key pest of mangoes and citrus from Texas to Costa Rica but the mechanisms of odorant perception in this species are poorly understood. Detection of volatiles in insects occurs mainly in the antenna, where molecules penetrate sensillum pores and link to soluble proteins in the hemolymph until reaching specific odor receptors that trigger signal transduction and lead to behavioral responses. Scrutinizing the molecular foundation of odorant perception in A. ludens< ...[more]

PMID: 33138264

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Project description:Mosquitoes are the vectors of a variety of infectious diseases. Olfaction and circadian rhythm gate different behavioral events in mosquitoes. However, the mechanisms of odor detection and processing of chemical signals, which are essential for olfactory perception, is poorly understood, including how perception changes in response to zeitgeber time in different mosquito species. To this end, we performed a circadian-dependent RNA-Sequencing study of the brain and peripheral olfactory-tissues of female Anopheles and Aedes mosquitoes. Data analysis revealed a significant upregulation of genes encoding: (a) chemosensory proteins (CSPs), required for transportation of odorant molecules towards the olfactory receptors, and; (b) xenobiotic-metabolizing enzymes (XMEs) during the dusk-transition phase. While XMEs primarily function in the elimination of toxic xenobiotics, concurrent elevation of XMEs and CSPs are hypothesized to act cumulatively for the regulation of perireceptor events and odorant sensitivity. During navigation towards their vertebrate-host/floral-host/mate-partners, female mosquitoes are exposed to diverse environmental chemicals of varied release rates that require continuous degradation/biotransformation by XMEs to avoid receptor saturation in such a noisy environment. Finally, our electroantennographic (EAG) analysis with both Anopheles and Aedes mosquitoes against diverse active volatile odorants, combined with XMEs inhibitors and RNAi studies, establish the proof-of-concept that XMEs function in peri-receptor events during odorant perception and influence the odorant sensitivity in mosquitoes. Additionally, our RNA-Seq and RNAi data also revealed that daily temporal modulation of neuronal serine proteases may facilitate the reorganization of synaptic connections influencing synaptic plasticity and olfactory perception in mosquitoes. In summary, this study highlights the crucial function of XMEs in odorant reception, therefore, improved understanding of species-specific rhythmic expression may provide an important information for successful implementation of mosquito control methods.

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	Ingel_0D_Female.xlsx	Xlsx
	Ingel_0D_Female_1.raw	Raw
	Ingel_0D_Female_2.raw	Raw
	Ingel_0D_Female_3.raw	Raw
	Ingel_0D_Female_4.raw	Raw

Dataset Information

Anastrepha ludens antenna proteome

Dataset's files

Publications

A First Glimpse of the Mexican Fruit Fly <i>Anastrepha ludens</i> (Diptera: Tephritidae) Antenna Morphology and Proteome in Response to a Proteinaceous Attractant.

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