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Aedes albopictus strain:embryos, larvae, pupae, adult males, sugar-fed and blood-fed adult female

ABSTRACT: embryos, larvae, pupae, adult males, sugar-fed and blood-fed adult female Transcriptome or Gene expression

PROVIDER: PRJNA178303 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR609260.fastq.gz	Fastqsanger.gz
	SRR609261.fastq.gz	Fastqsanger.gz
	SRR609263.fastq.gz	Fastqsanger.gz
	SRR609264.fastq.gz	Fastqsanger.gz
	SRR609265.fastq.gz	Fastqsanger.gz

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Aedes albopictus strain:embryos, larvae, pupae, adult males, sugar-fed and blood-fed adult female

Project description:embryos, larvae, pupae, adult males, sugar-fed and blood-fed adult female Transcriptome or Gene expression

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Project description:Chronic high-sugar feeding (1 M or 34% sucrose) leads to hyperglycemia, obesity, and insulin resistance in adult flies, compared with those fed a control diet (0.15 M or 5% sucrose). We compared two days and four weeks of high-sugar feeding to look at short- and long- term effects on gene expression. We used Affymetrix Drosophila GeneChip 2.0 microarrays to quantify differential expression between control and high-sugar-fed flies at two time points.

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Project description:Drosophila melanogaster undergoes a complete metamorphosis, during which time the larval male and female forms transition into sexually dimorphic, reproductive adult forms. To understand this complex morphogenetic process at a molecular-genetic level, we performed whole genome microarray analyses. Genes were identified that were expressed during metamorphosis in both somatic and germline tissues of males and females. Additionally, genes were identified that display sex-specific differences in abundance in both of these tissues at discrete times during metamorphosis. Keywords: time course; wild type; genetic modification; Gene expression was examined at five time points during metamorphosis: 0, 24, 48, 71, and 96 hr After Puparium Formation (APF). Gene expression was examined separately in males and females for both wild type pupae and tudor (tud) progeny. tud progeny have genetically ablated germline tissues. All samples were labeled with Cy5 and compared against a common reference sample labeled with Cy3. The reference sample contained male and female wild type pupae from all stages of metamorphosis. All experiments were conducted in triplicate.

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Whole body RNA-seq from developmental stages of Harpegnathos ants

Project description:RNA from Harpegnathos embryos, instar 1 larvae, instar 4 larvae, early pupae, late pupae, adult workers, and males was sequenced to look for gene expression changes throughout development.

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