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Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759

ABSTRACT: Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 Genome sequencing and assembly

PROVIDER: PRJNA207547 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	ATFJ01.dat.gz	Other
	ATFJ01.fasta.gz	Fasta.gz
	ATFJ01.master.dat	Other
	ATFJ01.dat.gz	Other

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Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759

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Genome sequencing of prophage-free variants of the fast growing, Gram negative bacteria Vibrio natriegens ATCC 14048

Project description:After the wild type strain of Vibrio natriegens ATCC 14048 (BioSample Nr. NCBI: SAMN03178087) was cured from prophages (as described in the material and methods section), the genomes of the resulted strains dvnp1, dvnp2 and vnp12 were sequenced. As a control, the genome of the wild type strain was also prepared and used for sequencing. The aim of the sequencing was to verify the deleted regions and to screen the genome for new mutations.

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Project description:Vibrio natriegens strain ATCC 14048 is a salt marsh isolate with a notably rapid growth rate that has garnered considerable interest for biotechnological applications. We used systems-level tools (i.e. metabolomics, transcriptomics, proteomics) to characterize its physiological response to different salinities and temperatures that are relevant not only to its natural environment but also to planned scalable culturing processes. We found organic osmolyte synthesis and membrane transporters were most responsive to changes in salinity. The osmolytes glutamate, glutamine and ectoine responded to salinity across temperature treatments. However, when media was supplemented with choline, glycine betaine appeared to replace ectoine. These results provide a baseline data set for metabolic activity under a variety of conditions that will influence future basic and applied V. natriegens research.

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RNA-seq validation experiments of prophage-free variants from Vibrio natriegens ATCC 14048

Project description:RNA-seq validation experiments of prophage-free variants from Vibrio natriegens ATCC 14048

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Project description:After the prophages of the Vibrio natriegens strain were deleted, RNA-seq experiments were conducted to obtain DGE-profiles in the presence and absence of the two prophages VNP1 and VNP2 in the exponential growth phase.

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