Project description:We report high-throughput profiling of genes bound by ULK3 and of H3K27 histone modification plus/minus ULK3 silencing in squamous cell carcinoma SCC13 cells .
Project description:<p>Polygonum minus Huds., commonly referred to as kesum, is a traditional medicinal plant in Malaysia known for its unique fragrance attributed to its volatile compounds. The essential oil extracted from <em>P. minus</em> displayed various benefits, comprised of antioxidant, antimicrobial and anticancer properties. Therefore, to identify volatile metabolites associated with such biological activities, we performed untargeted metabolite analysis on the essential oil extracted from <em>P. minus</em> leaf tissue. After the leaf samples were collected from the INBIOSIS experimental plot, a 6-hour hydro-distillation procedure was performed to extract the essential oil, which was then subjected to GC-MS analysis. Thus, this data could provide a reference and benchmark for researchers conducting analyses on volatile compounds in <em>P. minus</em>.</p>
Project description:A major virulence factor in S. enterica is the pathogenicity island Spi2. The goal of the experiment was to determine the impact of the toll-like receptor 4 (TLR-4) on expression of Spi2 in S. enterica wild type (WT) and triple mutant strain (TMS) with deletions in SPI-1 TTSS, SPI-2 TTSS, flagella. The stain 14028 was used for the experiment. Cells were grown in a TLR-4 wildtype (plus) or deleted (minus) macrophage background. Total RNA was extracted and processed by qRT-PCR.
Project description:A major virulence factor in S. enterica is the pathogenicity island Spi2. The goal of the experiment was to determine the impact of the NRAMP-1 (natural resistance-associated macrophage protein-1) on expression of Spi2 in S. enterica wild type (WT) and triple mutant strain (TMS) with deletions in SPI-1 TTSS, SPI-2 TTSS, flagella. The stain 14028 was used for the experiment. Cells were grown in a NRAMP-1 wildtype (plus) or deleted (minus) macrophage background. Total RNA was extracted and processed by qRT-PCR.
