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QRT-PCR of ex vivo Salmonella enterica in NRAMP-1 minus/plus macrophage background

ABSTRACT: A major virulence factor in S. enterica is the pathogenicity island Spi2. The goal of the experiment was to determine the impact of the NRAMP-1 (natural resistance-associated macrophage protein-1) on expression of Spi2 in S. enterica wild type (WT) and triple mutant strain (TMS) with deletions in SPI-1 TTSS, SPI-2 TTSS, flagella. The stain 14028 was used for the experiment. Cells were grown in a NRAMP-1 wildtype (plus) or deleted (minus) macrophage background. Total RNA was extracted and processed by qRT-PCR.

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S

PROVIDER: GSE26806 | GEO | 2011/06/13

SECONDARY ACCESSION(S): PRJNA135887

REPOSITORIES: GEO

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