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Phaeodactylum tricornutum

ABSTRACT: Next generation sequencing of Phaeodactylum tricornutum grown with and without nitrogen

PROVIDER: PRJNA242990 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1208033.fastq.gz	Fastqsanger.gz
	SRR1208034.fastq.gz	Fastqsanger.gz
	SRR1208035.fastq.gz	Fastqsanger.gz
	SRR1208036.fastq.gz	Fastqsanger.gz
	SRR1208037.fastq.gz	Fastqsanger.gz

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Next generation sequencing of Phaeodactylum tricornutum grown with and without nitrogen

2015-01-02 | GSE56346 | GEO

Next generation sequencing of Phaeodactylum tricornutum grown with and without nitrogen

Project description:We have done next generation sequencing of optically thin, exponentialy growing Phaeodactylum tricornutum cultures grown with and without nitrogen source to improve our undestanding of the pathways regulation under conditions that promote lipid accumulation (N-starvation). 3 biologically independent exponentially growing culture of Phaeodactylum tricornutum were pelleted and washed several times with N-free media. Each culture was devided to 2 replicates with initial cell concentration of 2X105 cells/mL, one with NaNO3 as nitrogen source and the other without any nitrogen source. The cuture were bubbled foro 48 hours and sampled for transcriptome together with other physiological parameters and lipid analysis.

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Next generation sequencing for understading the role of nitrate reductase in remodeling intermediate metabolism in Phaeodactylum tricornutum

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Next generation sequencing for understading the role of nitrate reductase in remodeling intermediate metabolism in Phaeodactylum tricornutum

Project description:We have done next generation sequencing of optically thin, exponentialy growing WT Phaeodactylum tricornutum and our ~50% nitrate reductase knock-down cultures. Culture were grown with and without nitrogen source to improve our undestanding of the pathways regulation under conditions that promote lipid accumulation (N-starvation). 3 biologically independent exponentially growing culture of the WT and the NR21 knock-down were pelleted and washed several times with N-free media. Each culture was devided to 2 replicates with initial cell concentration of 2.5X105 cells/mL, one with NaNO3 as nitrogen source and the other without any nitrogen source. The cuture were bubbled for 48 hours and sampled for transcriptome together with other physiological parameters and lipid analysis after reachign exponential growth.

2016-03-31 | E-GEOD-67449 | biostudies-arrayexpress

Mapping of H3K4me3 in the model diatom Phaeodactylum tricornutum

Project description:Chromatin organization in Phaeodactylum tricornutum

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Project description:Transcriptome of nitrogen starved and normal cultured Phaeodactylum tricornutum

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Project description:Phaeodactylum tricornutum Genome sequencing

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Early nutrient starvation response of the diatom Phaeodactylum tricornutum

Project description:An experiment was performed to determine the similarities on the RNA level between different conditions where cell division stops in the diatom Phaeodactylum tricornutum. Many of these conditions also increase the accumulation of lipids within the cell or impair photosynthesis. The different metabolic responses were evaluated and the dataset was mined for potential transcriptional regulators of these changes. The experimental setup was as follows: Cells from the pennate diatom Phaeodactylum tricornutum were grown in ESAW medium under continous fluorescent light at 21C in baffled shakeflasks. Exponentially growing cells were harvested by centrifugation and washed twice in 21gr/L NaCL to remove nutrients. Cells were subsequently resuspended in the five different media/conditions (control, darkness, no nitrate, no phosphate, nocodazole).

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Project description:Whole cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum

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Project description:Phaeodactylum tricornutum Raw sequence reads

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