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Glycine max

ABSTRACT: Gene Expression Changes in the Development of the Soybean Seed-Heart Stage

PROVIDER: PRJNA246314 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1272380.fastq.gz	Fastqsanger.gz
	SRR1272390.fastq.gz	Fastqsanger.gz
	SRR1272400.fastq.gz	Fastqsanger.gz
	SRR1272410.fastq.gz	Fastqsanger.gz
	SRR1272381.fastq.gz	Fastqsanger.gz

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Expression profiling of Glycine max seed maturation by high throughput sequencing

Project description:A transcriptome analysis of soybean seeds harvested at different developing stages (between stage 7.1 and stage 9) was carried out to understand the molecular events occuring during the acquisition of seed longevity during maturation.

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Metabolic and Transcriptional Reprogramming in Developing Soybean (Glycine max) Embryos

Project description:Soybean (Glycine max) seeds are an important source of seed storage compounds, including protein, oil, and sugar used for food, feed, chemical, and biofuel production. We assessed detailed temporal transcriptional and metabolic changes in developing soybean embryos to gain a systems biology view of developmental and metabolic changes and to identify potential targets for metabolic engineering. Two major developmental and metabolic transitions were captured enabling identification of potential metabolic engineering targets specific to seed filling and to desiccation. The first transition involved a switch between different types of metabolism in dividing and elongating cells. The second transition involved the onset of maturation and desiccation tolerance during seed filling and a switch from photoheterotrophic to heterotrophic metabolism. Clustering analyses of metabolite and transcript data revealed clusters of functionally related metabolites and transcripts active in these different developmental and metabolic programs. The gene clusters provide a resource to generate predictions about the associations and interactions of unknown regulators with their targets based on guilt-by-association relationships. The inferred regulators also represent potential targets for future metabolic engineering of relevant pathways and steps in central carbon and nitrogen metabolism in soybean embryos and drought and desiccation tolerance in plants.

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