Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Drosophila melanogaster

ABSTRACT: Expression from hemocytes misexpressing Idh-R195H vs. controls

OTHER RELATED OMICS DATASETS IN: E-GEOD-62008

PROVIDER: PRJNA262929 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

E-GEOD-62008

Project description:Expression from hemocytes misexpressing Idh-R195H vs. controls

2015-08-06 | E-GEOD-62008 | ExpressionAtlas

Expression from hemocytes misexpressing Idh-R195H vs. controls

Project description:Expression profile for hemocytes from hml-Gal4, UAS-2xEGFP larvae were compared to hemocytes from hml-Gal4, UAS-2xEGFP; UAS-Idh-R195H larvae We sought to determine which genes and pathways were upregulated or downregulated in hemocytes expressing Idh-R195H, the Drosophila homolog of the human cancer-associated IDH1-R132H mutant, in vivo. We sought to obtain homogeneous populations of hemocytes from each genotype. We compared larvae expressing Idh-R195H from the hml-Gal4 driver to larvae with the hml-Gal4 driver alone.

2014-10-03 | E-GEOD-62008 | biostudies-arrayexpress

Expression from hemocytes misexpressing Idh-R195H vs. controls

Project description:Expression profile for hemocytes from hml-Gal4, UAS-2xEGFP larvae were compared to hemocytes from hml-Gal4, UAS-2xEGFP; UAS-Idh-R195H larvae

2014-10-03 | GSE62008 | GEO

Drosophila melanogaster

Project description:Transcriptomic analysis of gene expression in adult Drosophila hemocytes

| PRJNA315975 | ENA

Drosophila melanogaster

Project description:The gene expression profiles in response to fungal infection in hemocytes.

| PRJNA107067 | ENA

Drosophila melanogaster lamellocytes proteome

Project description:In Drosophila melanogaster larval hemolymph, under normal conditions, plasmatocytes and crystal cells represent respectively ~95% and ~5% of hemocytes, while lamellocytes, the third larval cell type, are absent since they are only induced after parasitoid wasp oviposition, their role being the encapsulation-melanization response to eliminate the wasp egg. However, even after induction lamellocytes number remains low, making difficult biochemical studies. Here using the D. melanogaster hopTum-l mutant that constitutively produces a high number of hemocytes, we set up a method to purify lamellocytes and analyzed their major proteins by 2D gel electrophoresis and their biotinylated plasma membrane surface proteins by 1D SDS-PAGE after affinity purification. Mass spectrometry allowed to identify 430 proteins from the 2D spots and 344 from affinity purified proteins, totalizing 639 unique proteins. Known lamellocyte markers such as PPO3 and the integrin myospheroid are among the major proteins and affinity purification led to the detection of other integrins and a large array of integrins associated proteins involved in cell-cell junction formation and function. Overall newly identified proteins indicated that these cells are highly adapted to the encapsulation process but may have also several different physiological functions. This study provides the basis for new lamellocyte studies in vivo and in vitro, and develop markers to search whether different populations coexist, establish their origins and decipher their respective roles in drosophila physiology and immunity.

2024-04-29 | PXD016876 | Pride

Drosophila melanogaster

Project description:Drosophila melanogaster transcriptional profiling over life span: Indy vs. Controls

| PRJNA110479 | ENA

Drosophila melanogaster

Project description:Gene Expression Profiles of Drosophila third instar larvae hemocytes to explain hemocyte development.

| PRJNA107349 | ENA

Drosophila melanogaster

Project description:Gene expression profiling of endocycling cells vs mitotic cells

| PRJNA120627 | ENA

Drosophila melanogaster

Project description:GSC vs Kc