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Apopellia endiviifolia (species B)

ABSTRACT: Pellia endiviifolia (species B) overview

PROVIDER: PRJNA275022 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1407910.fastq.gz	Fastqsanger.gz
	SRR1407912.fastq.gz	Fastqsanger.gz
	SRR1408166.fastq.gz	Fastqsanger.gz
	SRR1408326.fastq.gz	Fastqsanger.gz
	SRR1408447.fastq.gz	Fastqsanger.gz

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Apopellia endiviifolia

Project description:Pellia endiviifolia overview

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Apopellia endiviifolia

Project description:Transcriptome analysis of the simple thalloid liverwort, Apopellia endiviifolia.

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Project description:High-throughput sequencing of endogenous small RNAs from the liverwort Pellia endiviifolia sp. B

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Chloroplast relocation movement in the liverwort Apopellia endiviifolia.

Project description:Changes in the subcellular localisation of chloroplasts help optimise photosynthetic activity under different environmental conditions. In many plants, this movement is mediated by the blue-light photoreceptor phototropin. A model organism with simple phototropin signalling that allows clear observation of chloroplasts would facilitate the study of chloroplast relocation movement. Here, we examined this process in the simple thalloid liverwort Apopellia endiviifolia. Transverse sections of the thallus tissue showed uniformly developed chloroplasts and no air chambers; these characteristics enable clear observation of chloroplasts and analysis of their movements under a fluorescence stereomicroscope. At 22°C, the chloroplasts moved to the anticlinal walls of cells next to the neighbouring cells in the dark (dark-positioning response), whereas they moved towards weak light (accumulation response) and away from strong light (avoidance response). When the temperature was reduced to 5°C, the chloroplasts moved away from weak light (cold-avoidance response). Hence, both light- and temperature-dependent chloroplast relocation movements occur in A. endiviifolia. Notably, the accumulation, avoidance and cold-avoidance responses were induced under blue-light but not under red-light. These results suggest that phototropin is responsible for chloroplast relocation movement in A. endiviifolia and that the characteristics are similar to those in the model liverwort Marchantia polymorpha. RNA sequencing and Southern blot analysis identified a single copy of the PHOTOTROPIN gene in A. endiviifolia, indicating that a simple phototropin signalling pathway functions in A. endiviifolia. We conclude that A. endiviifolia has great potential as a model system for elucidating the mechanisms of chloroplast relocation movement.

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Nucleosome positioning for 4 yeast species

Project description:We describe the genome-wide nucleosome profiles of four related yeast species. All species display the same global organization features first described in S. cerevisiae: a stereotypical nucleosome organization along genes, and the classification of promoters into these which contain or lack a pronounced Nucleosome Depleted region (NDR), with the latter displaying a more dynamic pattern of gene expression. This global similarity, however, does not reflect a static evolutionary pattern, as nucleosome positioning at specific genes diverged rapidly leaving practically no similarity between S. cerevisiae and C. glabrata orthologs (~50 Myr). We show that this rapid divergence in nucleosome positioning contrasts a conserved pattern of gene expression, consistent with the idea that divergence of nucleosome patterns has a limited effect on gene expression as many different configurations can support the same regulatory outcome. Nucleosomes from 4 different yeast species were isolated and sequenced using the Illumina GAII platform. Replicates were performed for 3 of the species

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A gDNA microarray for genotyping Salvia species

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Project description:This study aims to compar transcription factor binding region between five mouse species.

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