Project description:Ribosome profiling (Ribo-Seq) (maps positions of translating ribosomes on the transcriptome) and RNA-Seq (quantifies the transcriptome) analysis of Rattus norvegicus cells infected with Moloney Murine Leukemia Virus (Mo-MuLV).
Project description:Histone modifcations and CTCF binding at the c-myb locus were compared in cell lines with c-myb expressing, which are myeloblatic M1 cells and leukemia cells with virus integration, VS. M1 cells without c-myb expression induced by IL-6. Distribution of active histone marks at the c-myb gene and the upstream regions are associated with active c-myb transcription. The enrichment of all of these active histone marks decreased with differentiation-induced down-regulation of c-myb, but increased and spread in tumor cells. ChIP-on-chip from murine myeloid cell line M1 and virus-induced myeloid leukemia cell lines for H3K4me3, H3K9/14ac, H3K4me1, H3K27me3, H3K9me3 and CTCF
Project description:Single-cell RNA-seq analysis of cortex and hippocampus tissue from murine males and females after recovery from West Niles virus encephalitis
Project description:Infection of cells with murine hepatitis virus strain A59 (MHV-A59) results in massive amounts of viral RNA within infected cells. When applying transcriptional profiling by means of microarray analysis, this could potentially cause problems since high amounts of viral RNA could in theory interfere with the analysis. Since coronavirus RNAs are polyadenylated, the viral RNAs are also amplified by oligo(dT) primers (a standard procedure when processing RNA samples for array hybridization). In this experiment we compared two different array platforms for the potential interference of viral RNA, using MHV-A59 infection of LR7 (murine fibroblasts) as a model.
Project description:Transcriptional profiling of blood B cells from bovine leukemia virus-infected cattle comparing IgMhigh B cells with IgMlow B cells. Goal was to estimate the difference of cellular function in both subset. Two-condition experiment, IgMhigh B cells vs. IgMlow B cells from three bovine leukemia virus-infected cattle.
Project description:Ezh2 and EZH1 are histone H3 lysine 27 (H3K27)-specific methyltransferases. Their hyperactive mutations and overexpression were found in cancer including various hematological malignancies. UNC1999 is a highly selective inhibitor for both enzymes. It suppresses H3K27 tri- and di-methylation globally and inhibits growth of MLL-rearranged acute leukemia. Here we performed ChIP-Seq to profile how UNC1999 affects distribution of H3K27me3 and its antagonizing H3K27ac in MLL-AF9-immortalized leukemia cells. We also performed ChIP-seq of SUZ12, an essential common cofactor of EZH2 and EZH1 following compound treatments. We treated MLL-AF9 transformed murine leukemia cells with DMSO, UNC1999 or UNC2400 (an inactive analog compound of UNC1999). Cells were then collected and used for ChIP-Sequencing of Input, H3K27me3, SUZ12, and H3K27ac.
