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Survey of methylomes of two beetles, Nicrophorus vespilloides and Tribolium castaneum

ABSTRACT: Survey of methylomes of two beetles, Nicrophorus vespilloides and Tribolium castaneum

PROVIDER: PRJNA283826 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2017560.fastq.gz	Fastqsanger.gz
	SRR2017555.fastq.gz	Fastqsanger.gz
	SRR2017556.fastq.gz	Fastqsanger.gz
	SRR2017557.fastq.gz	Fastqsanger.gz
	SRR2017558.fastq.gz	Fastqsanger.gz

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Survey of methylomes of two beetles, Nicrophorus vespilloides and Tribolium castaneum

Project description:The presence of DNA methylation in beetles (Coleoptera) has only been investigated with bisulfite sequencing of Tribolium castaneum, which produced no evidence of DNA methylation. Here, we used whole genome bisulfite sequencing to assay if DNA methylation was present in another beetle, Nicrophorus vespilloides. We used T. castaneum as a negative control.

2015-10-21 | GSE68814 | GEO

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Nicrophorus vespilloides

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Deep sequencing of small RNAs from Tribolium castaneum

Project description:Characterization of miRNAs in red flour beetle Tribolium castaneum by deep sequencing of two different RNA libraries. Sequencing of Tribolium small RNAs from adults and embryos.

2010-12-29 | E-GEOD-26036 | biostudies-arrayexpress

Deep sequencing of small RNAs from Tribolium castaneum

Project description:Characterization of miRNAs in red flour beetle Tribolium castaneum by deep sequencing of two different RNA libraries.

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Identification of maternally-loaded RNA transcripts in unfertilized eggs of Tribolium castaneum

Project description:Using a tiled whole-genome microarray, we found that 58.2% of Tribolium castaneum genes are maternally loaded into eggs. Comparison of known Drosophila melanogaster maternal genes to our results showed widespread conservation of maternal function with T. castaneum. We also found many T. castaneum genes with previously identified gender or tissue specific expression were also maternally loaded into eggs. The microarray design also allowed the detection of 2315 and 4060 novel transcriptionally active regions greater in length than 100 bp in unfertilized and fertilized T. castaneum eggs, respectively. The primary objective of this study was to identify expressed regions of the Tribolium castaneum genome in unfertilized and fertilized eggs using a whole-genome tiled microarray. The whole RNA of 3 samples of virgin laid eggs and 3 samples of fertilized eggs were compaired.

2013-05-06 | E-GEOD-38245 | biostudies-arrayexpress

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