Project description:description Blastocystis sp. is a highly prevalent anaerobic eukaryotic parasite of humans and animals. The genome of several representatives has been sequenced revealing specific traits such as an intriguing 3’-end processing of primary transcripts. We have acquired a first high-throughput proteomics dataset on the difficult to cultivate ST4 isolate WR1 and detected 2,761 proteins. We evidenced for the first time by proteogenomics a functional termination codon derived from transcript polyadenylation for seven different key cellular components.
Project description:To assess the significant downstream pathways affected by the overexpression of CsubMADS1 in Coccomyxa, transcriptome analysis was carried out using RNA sequencing of three biological replicates each from WT and CsubMADS1 OX at stationary phase (3h before dark phase). The stationary phase was chosen for the analysis because this is when the nutrients start depleting in the medium and starvation stress is induced.
