ABSTRACT: Comparing transcriptome by means of DNA microarrays of Streptococcus pneumoniae D39 grown with mannose to cells grown with the same sugar after a 1 hr pulse with glucose
Project description:Comparing transcriptome by means of DNA microarrays of Streptococcus pneumoniae D39 grown with galactose to cells grown with the same sugar after a 1 hr pulse with glucose
Project description:Comparing transcriptome by means of DNA microarrays of Streptococcus pneumoniae D39 grown with N-acetylglucosamine to cells grown with the same sugar after a 1 hr pulse with glucose
Project description:Comparing transcriptome by means of DNA microarrays of Streptococcus pneumoniae D39 grown with a mix of sugars (mannose, N-acetylglucosamine, galactose) to cells grown with the same mix after a 1 hr pulse with glucose
Project description:Comparing transcriptome by means of DNA microarrays of Streptococcus pneumoniae D39 grown with mannose to cells grown with glucose as carbon source
Project description:Comparing transcriptome by means of DNA microarrays of Streptococcus pneumoniae D39 grown with galactose to cells grown with glucose as carbon source
Project description:Comparing transcriptome by means of DNA microarrays of Streptococcus pneumoniae D39 grown with N-acetyl glucosamine to cells grown with glucose as carbon source
Project description:This study addresses the response of Streptococcus pneumoniae to zinc. The wild-type strain D39 was grown in GM17 and GM17 with 0.25 mM of ZnSO4. The transcriptome under these conditions was compared by using microarrays. Keywords: transcriptome analysis of D39 wild-type compared with its isogenic glnPA double mutant
Project description:Comparison of the Streptococcus pneumoniae D39 spxB- mutant vs D39. For DNA microarray analysis, D39 wild-type and two independently obtained D39spxB- mutants were grown as four, two and two biological replicates, respectively, in Glc-M17 under semi-aerobic conditions and harvested at an OD595 of approximately 0.25 (mid-exponential). The RNA of both D39spxB- strains was compared to D39 and the combined data was analyzed. All other procedures regarding microarray analyses were done as described before. A gene was considered differentially expressed when the fold change was ≥ 2, ≤ 0.5, with a Bayes p ≤ 0.00001 and when at least 7 measurements were available.
