Project description:This project seeks to identify protein-protein interactions with human endogenous retrovirus K envelope protein. A flag-tagged HERV-K env construct (fHERV-K env) was expressed in HEK293 cells. An anti-flag MAb was immobilized on magnetic protein G beads was used to capture fHERV-K env and interactors.
Project description:We surveyed RNA-Seq data to identify those TEs that are transcriptionally active uniquely in human pluripotent cells. We identified one endogenous retrovirus (HERV-H) family, uniquely found in primates as being unusually abundant in the transcriptome.
