Proteomics

Dataset Information

0

Co-IP of fHERV-K env protein and interactors


ABSTRACT: This project seeks to identify protein-protein interactions with human endogenous retrovirus K envelope protein. A flag-tagged HERV-K env construct (fHERV-K env) was expressed in HEK293 cells. An anti-flag MAb was immobilized on magnetic protein G beads was used to capture fHERV-K env and interactors.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Jeffrey Kowalak  

LAB HEAD: Jeffrey A Kowalak

PROVIDER: PXD019702 | Pride | 2020-07-06

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
13OCT25_01.mgf Mgf
13OCT25_01.raw Raw
13OCT25_02.mgf Mgf
13OCT25_02.raw Raw
13OCT28_01.mgf Mgf
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Publications

Regulation of stem cell function and neuronal differentiation by HERV-K via mTOR pathway.

Wang Tongguang T   Medynets Marie M   Johnson Kory R KR   Doucet-O'Hare Tara T TT   DiSanza Brianna B   Li Wenxue W   Xu Yadi Y   Bagnell Anna A   Tyagi Richa R   Sampson Kevon K   Malik Nasir N   Steiner Joseph J   Hadegan Alina A   Kowalak Jeffrey J   O'Malley James J   Maric Dragan D   Nath Avindra A  

Proceedings of the National Academy of Sciences of the United States of America 20200715 30


Stem cells are capable of unlimited proliferation but can be induced to form brain cells. Factors that specifically regulate human development are poorly understood. We found that human stem cells expressed high levels of the envelope protein of an endogenized human-specific retrovirus (HERV-K, HML-2) from loci in chromosomes 12 and 19. The envelope protein was expressed on the cell membrane of the stem cells and was critical in maintaining the stemness via interactions with CD98HC, leading to t  ...[more]

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