Project description:Analysis of the transcriptional response of mouse embryonic fibroblasts to reovirus infection. The hypothesis tested was that both peroxisomal and mitochondrial MAVS is capable of inducing the expression of antiviral genes. mRNA isolated from MAVS-expressing cells after infection for various times with reovirus
Project description:Packaging of segmented, double-stranded RNA viral genomes requires coordination of multiple viral proteins and RNA segments. For mammalian orthoreovirus (reovirus), evidence suggests either all ten or zero viral RNA segments are simultaneously packaged in a highly coordinated process hypothesized to exclude host RNA. Accordingly, reovirus generates genome-containing virions and “genomeless” top component particles. However, despite ostensibly lacking the genome, top component particles maintain a low level of infectivity. Whether reovirus particles can package host RNA is unknown. To gain insight into reovirus packaging potential and mechanisms, we employed next-generation RNA-sequencing to define the viral and host RNA content of purified reovirus virions and top component particles. Reovirus top component particles contained double-stranded viral RNA segments in similar proportions but at reduced levels compared to virions. Top component particles also were enriched for numerous host RNAs, especially short, non-polyadenylated transcripts, that differed by reovirus strain, independent of the viral polymerase. In contrast, virions were enriched for very few host RNAs. Collectively, these findings indicate that genome packaging into reovirus virions is exquisitely selective, while incorporation of host RNAs into top component particles is more promiscuous or differentially selective and may contribute to or result from inefficient viral RNA packaging.
Project description:Reovirus mediated cell death of breast cancer is orchestrated via apoptotic cell death pathways We used inhouse microarrays to detail the global programme of gene expression following reovirus treatment
Project description:Analysis of reovirus-induced host responses at the level of gene expression. Results provide insights into how reovirus infection breaks tolerance to dietary antigens and promotes the development of celiac disease.
Project description:Analysis of reovirus-induced host responses at the level of gene expression. Results provide insights into how reovirus infection breaks tolerance to dietary antigens and promotes the development of celiac disease.
Project description:We utilized oligonucleotide microarrays to measure cellular mRNA decay rates in mock- or reovirus-infected murine L929 cells to determine if changes in host mRNA expression are a consequence of reovirus-induced alterations in cellular mRNA stability.
