Project description:Histone acetylations are known to impact gene transcription. Here, we have attempted to examine the role of Leishmania donovani histone acetyltransferases HAT4 and HAT2 in regulating global gene expression. The transcriptome of HAT4-null promastigotes (Samples 2A, 2B) and HAT2-heterozygous knockout promastigotes (Samples 3A, 3B) have been compared with the transcriptome of wild-type Leishmania promastigotes (Samples 1A, 1B) and fold change in gene expression with respect to the control wild-type has been determined.
Project description:Monastrol treatment of Leishmania donovani infected macrophages Macrophages were infected with Leishmania donovani and treated with monastrol to look for signalling molecules
Project description:The mRNA expression of antimony resistant strains of Leishmania donovani was compared to the expression of the sensitive Leishmania donovani.
Project description:The mRNA expression of antimony resistant strains of Leishmania donovani was compared to the expression of the sensitive Leishmania donovani. The antimony resistant and sensitive Leishmania donovani were grown in complete M199 medium with 10% FCS and Penicillin streptomycin mixture. At stationary phase (5 day culture) cells were harvested in sterile Phosphate buffered saline and used for RNA isolation.
Project description:Gene expression analysis was carried out between experimental artesunate resistant Leishmania donovani (adapted to 50 µM artesunate drug pressure) and it's corresponding wild type artesunate sensitive parasite.
Project description:Gene expression analysis was carried out between experimental Paromomycin resistant Leishmania donovani (adapted to 100 µM paromomycin drug pressure) and it's corresponding wild type paromomycin sensitive parasite.
Project description:Differential gene expression analysis was carried out in clinical isolates of Leishmania donovani (L. donovani) parasite derived from VL patient before miltefosine treatment, post treatment and patient that relpased after miltefosine treatment
