Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bombus terrestris

ABSTRACT: A MicroRNA Associated With Caste Determination in a Bumblebee is Expressed from a Mirtron Within a Homologue of Vitellogenin

PROVIDER: PRJNA311930 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR3166830.fastq.gz	Fastqsanger.gz
	SRR3166823.fastq.gz	Fastqsanger.gz
	SRR3166824.fastq.gz	Fastqsanger.gz
	SRR3166825.fastq.gz	Fastqsanger.gz
	SRR3166826.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Proteomic analysis of Bombus terrestris queen haemolymph

Project description:Enabled by recently available genomic resources for bumblebees, nano LC-MS/MS based proteomics was conducted to characterise the queen haemolymph proteome. The resulting mass spectrometry data were also utilised in a proteogenomic capacity to identify proteins missing from official gene sets and to improve gene models and annotation. 97 multiple-peptide supported proteins within queen bumblebee haemolymph were identified including 28 immunity-related proteins with roles in immune recognition, signalling and regulation. In addition 19 proteins with potential immune roles were identified. The haemolymph proteome is particularly rich in metabolism and transport proteins and comprised 18 proteins with unknown function.

2015-02-24 | PXD001644 | Pride

Bombus terrestris

Project description:The effect of DNA methylation on bumblebee colony development

| PRJNA638377 | ENA

Bombus terrestris

Project description:Strain-level analysis reveals the vertical microbial transmission during the life cycle of bumblebee

| PRJNA705073 | ENA

Bombus terrestris audax

Project description:Evolution and molecular basis of caste differentiation in bees

| PRJNA355856 | ENA

Bombus terrestris audax

Project description:Caste- and pesticide-specific effects of neonicotinoid pesticide exposure on gene expression in bumblebees

| PRJNA508397 | ENA

A MicroRNA Associated With Caste Determination in a Bumblebee is Expressed from a Mirtron Within a Homologue of Vitellogenin

Project description:Our aim was to use next-generation sequencing to identify the miRNAs associated with caste determination in queen-destined and worker-destined larvae of the bumble bee, Bombus terrestris. We found that two miRNAs (miR-6001-5p and miR-6001-3p) were upregulated in queen-destined larvae that had passed the critical developmental period when caste becomes irreversible in this species. The two miRNAs form a duplex that is expressed from the gene VHDL, a homologue of Vitellogenin. This finding suggests a new connection between miRNAs and a key protein known to play multiple roles in regulating reproductive division of labour in eusocial Hymenoptera .

2017-03-31 | GSE77870 | GEO

Honey proteome of the bumblebee Bombus terrestris

Project description:The biological samples analyzed in this study were honey samples from the bumblebee B. terrestris. Commercially available Tripol hives used for pollination consist of three complete bumblebee colonies (Koppert Biological Systems). Four Tripol hives (12 colonies overall) were placed in the Crop Research Institute (Prague-Ruzyne, Czechia) at the time of rapeseed Brassica napus and apple tree flowering. Honey stores were manually collected into 50-mL sterile centrifuge tubes. Honey samples per colony were pooled. These 12 honey samples were analyzed using label-free nano-LC-MS/MS. In addition, 3 bands excised from 1D-E SDS-PAGE were analyzed using nano-LC-MS/MS. Note that modification for analysis the bands was carbamidomethyl instead of MethylThio. Combined.txt and used databases are provided.

2022-03-03 | MSV000088979 | MassIVE

MiRNAs in the genomes of Bombus terrestris and Bombus impatiens

Project description:Our aims in this study were: 1) to identify the miRNAs of the bumble bees Bombus terrestris and B. impatiens; 2) to compare the total numbers of miRNAs between both bumble bee species and between them and the honey bee, Apis mellifera; and 3) to test whether the sequences and expression patterns of miRNAs were conserved between species. To investigate each of these aims we used miRNA-seq (deep sequencing of miRNA-enriched libraries) in B. terrestris, and bioinformatics prediction programs to identify miRNAs in both Bombus species. We identified 131 miRNAs in B. terrestris, and 114 in B. impatiens; of these, 17 were new miRNAs that had not previously been sequenced in any species. We found a striking level of difference in the miRNAs present between Bombus and A. mellifera, with 103 miRNAs in A. mellifera not being present in the genomes of the two bumble bees.

2015-03-06 | GSE64512 | GEO

Bombus terrestris RNA-seq data

Project description:RNA-seq data of 27 combinations of caste and developmental stage for Bombus terrestris.

| PRJEB9366 | ENA