Ambystoma mexicanum
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA315425 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:While considerable progress has been made towards understanding the complex processes and pathways that regulate human wound healing, regenerative medicine has been unable to develop therapies that coax the natural wound environment to heal scar-free. The inability to induce perfect skin regeneration stems partly from our limited understanding of how scar-free healing occurs in a natural setting. Here we have investigated the wound repair process in adult axolotls and demonstrate that they are capable of perfectly repairing full thickness excisional wounds made on the flank. In the context of mammalian wound repair, our findings reveal a substantial reduction in hemostasis, reduced neutrophil infiltration and a relatively long delay in production of new extracellular matrix (ECM) during scar-free healing. Additionally, we test the hypothesis that metamorphosis leads to scarring and instead show that terrestrial axolotls also heal scar-free, albeit at a slower rate. Analysis of newly forming dermal ECM suggests that low levels of fibronectin and high levels of tenascin-C promote regeneration in lieu of scarring. Lastly, a genetic analysis during wound healing comparing epidermis between aquatic and terrestrial axolotls suggests that matrix metalloproteinases may regulate the fibrotic response. Our findings outline a blueprint to understand the cellular and molecular mechanisms coordinating scar-free healing that will be useful towards elucidating new regenerative therapies targeting fibrosis and wound repair. We used microarray analysis to determine the gene expression changes that take place during scar free wound healing in aquatic and terrestrial axolotl salamanders. Epidermal tissue was harvested using a 4mm biopsy punch. Two wounds were made along the flank and posterior to the forelimbs. Harvested epidermis was pooled for each animal. Four biological replicates were collected from uninjured epidermis (D0) and at 1, 3, and 7 days post injury.
2012-01-31 | E-GEOD-35255 | biostudies-arrayexpress
Project description:Salamanders, such as the Mexican axolotl, are some of the few vertebrates fortunate in their ability to regenerate diverse structures after injury. Unlike mammals they are able to regenerate a fully functional spinal cord after injury. Throughout human life, many cells and certain tissues, such as hair follicle stem cells and liver, can be continuously replaced to maintain functional integrity in response to normal daily wear and tear. However, the human response to more serious tissue damage is limited to relatively primitive wound healing, whereby collagenous scar tissue fills the injury site, assuring the tissue’s structural integrity but often resulting in a debilitating loss of functionality. In contrast many vertebrates, including axolotls, have remarkable regenerative capacity including the functional regeneration of full thickness wounds. Here, we have identified a novel role for SALL4 in regulating collagen transcriptional after injury that is essential to ensure perfect skin regeneration in axolotl.
2017-03-07 | GSE79299 | GEO
Project description:Identification of conserved and novel microRNAs during tail regeneration in the Mexican Axolotl
| PRJNA292834 | ENA
Project description:Expression data from regenerating axolotl forelimbs
| PRJNA360578 | ENA
Project description:While considerable progress has been made towards understanding the complex processes and pathways that regulate human wound healing, regenerative medicine has been unable to develop therapies that coax the natural wound environment to heal scar-free. The inability to induce perfect skin regeneration stems partly from our limited understanding of how scar-free healing occurs in a natural setting. Here we have investigated the wound repair process in adult axolotls and demonstrate that they are capable of perfectly repairing full thickness excisional wounds made on the flank. In the context of mammalian wound repair, our findings reveal a substantial reduction in hemostasis, reduced neutrophil infiltration and a relatively long delay in production of new extracellular matrix (ECM) during scar-free healing. Additionally, we test the hypothesis that metamorphosis leads to scarring and instead show that terrestrial axolotls also heal scar-free, albeit at a slower rate. Analysis of newly forming dermal ECM suggests that low levels of fibronectin and high levels of tenascin-C promote regeneration in lieu of scarring. Lastly, a genetic analysis during wound healing comparing epidermis between aquatic and terrestrial axolotls suggests that matrix metalloproteinases may regulate the fibrotic response. Our findings outline a blueprint to understand the cellular and molecular mechanisms coordinating scar-free healing that will be useful towards elucidating new regenerative therapies targeting fibrosis and wound repair.
2012-01-31 | GSE35255 | GEO
Project description:axolotl 10x single cell RNA Raw sequence reads
| PRJNA589484 | ENA
Project description:Microarray Analysis of microRNA Expression during Axolotl Limb Regeneration
| PRJNA141103 | ENA
Project description:Small RNA and mRNA expression profiling during axolotl forelimb regeneration
| PRJNA299879 | ENA
Project description:Gene Expression During the First 28 Days of Axolotl Limb Regeneration
| PRJNA278991 | ENA
Project description:Differentially expressed genes during axolotl jaw regeneration identified by RNA-seq
| PRJNA376733 | ENA