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Streptococcus agalactiae A909

ABSTRACT: Streptococcus agalactiae A909 Targeted loci cultured

PROVIDER: PRJNA315444 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3234240_1.fastq.gz	Fastqsanger.gz
	SRR3234240_2.fastq.gz	Fastqsanger.gz
	SRR3234241_1.fastq.gz	Fastqsanger.gz
	SRR3234241_2.fastq.gz	Fastqsanger.gz
	SRR3234242_1.fastq.gz	Fastqsanger.gz

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RNA-seq of a Streptococcus agalactiae A909 mutant strain deleted for the pleiotropic transcriptional regulator CcpA

Project description:The commensal bacterium Streptococcus agalactiae is responsible for various infections in a wide variety of hosts including humans. Its broad spectrum of hosts shows its ability to acquire nutrients in variable conditions. The carbon catabolite repression allows bacteria to prioritize the uptake and the catabolism of the environmental sugars. In Gram-positive bacteria, CcpA (catabolite control protein A), a pleiotropic transcriptional regulator, plays a key role in catabolite repression. Studies have shown the involvement of carbon catabolite repression in the adaptation and stress resistance of pathogenic bacteria. The goal of this study is to determine the regulon and the role(s) of CcpA in the physiology and adaptation of S. agalactiae. To this aim, Streptococcus agalactiae strain A909 WT and its isogenic mutant ∆ccpA, obtained by allelic exchange were grown in filter-sterilized chemically defined medium (CDM) supplemented with 0,25% or 1% (w/v) of glucose. Their transcriptomes were compared under these two conditions by using RNA-seq.

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Stp1 regulated genes in Streptococcus agalactiae strain A909

Project description:Genes that showed altered expression between wild type A909 and the isogenic Stp1 mutant were identified by microarray analysis

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Transcriptome comparison between S. agalactiae strains SS1219 and A909

Project description:S. agalactiae strains A909 (ST7 isolated from human) and SS1219 (ST260 isolated from fish) were grown in TH medium at 30C and harvested at OD 0.3-0.4

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RgfC/A regulated genes in Streptococcus agalactiae strain A909

Project description:Genes that showed altered expression between wild type A909 and isogenic rgfC/A mutant were identified. A strain with a deletion in RgfC/A (SAK_1917/SAK_1918) was derived and microarray analysis was performed to identify genes regulated by the RgfC/A two component system. strain comparison, global regulation, two component system

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Transcriptome comparison between S. agalactiae strains 2-22 and A909

Project description:Strains 2-22 (S. agalactiae ST261 isolated from fish) and A909 (ST7) were grown in TH medium, at 30C and harvested at OD 0.3-0.4. Please note: ST261 and ST7 refer to MLST types commonly used in S.agalactiae as a first approach for phylogenomic relationships (MLST is based on the sequence of 7 genes).

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RgfC/A regulated genes in Streptococcus agalactiae strain A909

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Stp1 regulated genes in Streptococcus agalactiae strain A909

Project description:Genes that showed altered expression between wild type A909 and the isogenic Stp1 mutant were identified by microarray analysis Deletion mutants were examined for changes in gene expression

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Project description:Stp1 regulated genes in Streptococcus agalactiae strain A909

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Streptococcus agalactiae A909

Project description:Streptococcus agalactiae Tn-seq after outgrowth in human blood

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