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Streptococcus agalactiae A909

ABSTRACT: Streptococcus agalactiae Tn-seq after outgrowth in human blood

PROVIDER: PRJNA416503 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6253002.fastq.gz	Fastqsanger.gz
	SRR6253003.fastq.gz	Fastqsanger.gz
	SRR6253004.fastq.gz	Fastqsanger.gz
	SRR6253005.fastq.gz	Fastqsanger.gz
	SRR6253006.fastq.gz	Fastqsanger.gz

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RNA-seq of a Streptococcus agalactiae A909 mutant strain deleted for the pleiotropic transcriptional regulator CcpA

Project description:The commensal bacterium Streptococcus agalactiae is responsible for various infections in a wide variety of hosts including humans. Its broad spectrum of hosts shows its ability to acquire nutrients in variable conditions. The carbon catabolite repression allows bacteria to prioritize the uptake and the catabolism of the environmental sugars. In Gram-positive bacteria, CcpA (catabolite control protein A), a pleiotropic transcriptional regulator, plays a key role in catabolite repression. Studies have shown the involvement of carbon catabolite repression in the adaptation and stress resistance of pathogenic bacteria. The goal of this study is to determine the regulon and the role(s) of CcpA in the physiology and adaptation of S. agalactiae. To this aim, Streptococcus agalactiae strain A909 WT and its isogenic mutant ∆ccpA, obtained by allelic exchange were grown in filter-sterilized chemically defined medium (CDM) supplemented with 0,25% or 1% (w/v) of glucose. Their transcriptomes were compared under these two conditions by using RNA-seq.

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