Project description:Our objective was to identify candidate genes that contribute to the long 31-hour circadian period previously observed in DGRP_892. We performed transcriptional profiling of whole fly heads from two genotypes: DGRP_892, and Canton-S B, a line with a normal 24-hour circadian period. We collected fly heads every two hours over a 24-hour period. We quantified differential expression among genotype, time, and sex.
Project description:We applied ribosomal footprinting experiment followed by RNA-seq in order to detect circular RNA associated with ribosomes in fly heads.
Project description:To measure the response to gene dose, we performed mRNA-Seq of fly heads with molecularly defined deletions constructed from DrosDel deficiency lines (Ryder et al. Genetics 2007, 177(1):615-29) on the Illumina HiSeq 2000 platform.
Project description:Strychnine (STR) is a convulsant agent. In an effort to develop a Drosophila model of kindling epileptogenesis, we have examined chronic STR induced alterations in gene expression profile of fly heads. Expression profiling of fly heads after 7 day long STR treatment revealed perturbations in various functional category of genes. Keywords: Drug Response
Project description:Pilocarpine hydrochloride (PILO) is an epileptogenic agent. In an effort to develop a Drosophila model of kindling epileptogenesis, we have examined chronic PILO induced alterations in gene expression profile of fly heads. Expression profiling of fly heads after 7 day long PILO treatment revealed perturbations in various functional category of genes. Keywords: Drug Response
Project description:Tetraethylammonium chloride (TEA) is an epileptogenic agent. In an effort to develop a Drosophila model of kindling epileptogenesis, we have examined chronic TEA induced alterations in gene expression profile of fly heads. Expression profiling of fly heads after 7 day long TEA treatment revealed perturbations in various functional category of genes. Keywords: Drug Response
